Synthetic Biology and Metabolic Engineering of Microalgae for Sustainable Lipid and Terpenoid Production: An Updated Perspective.

IF 10.5 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Plant Biotechnology Journal Pub Date : 2025-10-21 DOI:10.1111/pbi.70405

Ty Shitanaka,Yu Wang,Sally Do,Julia Yuson,Samir Kumar Khanal,Krzysztof Zienkiewicz,Zhi-Yan Du

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引用次数: 0

Abstract

Microalgae are increasingly recognised as powerful platforms for the sustainable production of lipids and terpenoids, with expanding applications in the food, fuel and biomanufacturing industries. In this updated review, we consolidate and critically assess the most recent advances in synthetic biology and metabolic engineering of key microalgal models, including Chlamydomonas reinhardtii, Nannochloropsis spp. and Phaeodactylum tricornutum. We focus on developments that have emerged in the latest waves of research, emphasising novel genetic toolkits that accelerate the Design-Build-Test-Learn (DBTL) cycle, breakthroughs in genome-scale metabolic modelling, and innovative strategies for organelle-targeted biosynthesis of high-value compounds. Recent case studies are compared to highlight trends in successful engineering approaches. By capturing these up-to-date insights, this review outlines the current trajectory of microalgal biotechnology toward scalable, carbon-neutral biofactories for polyunsaturated fatty acids (PUFAs) and diverse terpenoids, reinforcing their role in global sustainability and the circular bioeconomy.

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微藻合成生物学和代谢工程对脂类和萜类可持续生产的最新研究进展。

微藻越来越被认为是可持续生产脂类和萜类的强大平台，在食品、燃料和生物制造行业的应用越来越广泛。在这篇最新的综述中，我们整合并批判性地评估了主要微藻模型的合成生物学和代谢工程的最新进展，包括莱茵衣藻、纳米绿藻和三角褐指藻。我们专注于最新研究浪潮中出现的发展，强调加速设计-构建-测试-学习（DBTL）周期的新型遗传工具包，基因组尺度代谢建模的突破，以及高价值化合物的细胞器靶向生物合成的创新策略。最近的案例研究进行比较，以突出成功的工程方法的趋势。通过捕捉这些最新的见解，本文概述了微藻生物技术朝着可扩展的、碳中性的多不饱和脂肪酸（PUFAs）和多种萜类生物工厂发展的当前轨迹，加强了它们在全球可持续性和循环生物经济中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Biotechnology Journal 生物-生物工程与应用微生物

CiteScore

20.50

自引率

2.90%

发文量

201

审稿时长

1 months

期刊介绍： Plant Biotechnology Journal aspires to publish original research and insightful reviews of high impact, authored by prominent researchers in applied plant science. The journal places a special emphasis on molecular plant sciences and their practical applications through plant biotechnology. Our goal is to establish a platform for showcasing significant advances in the field, encompassing curiosity-driven studies with potential applications, strategic research in plant biotechnology, scientific analysis of crucial issues for the beneficial utilization of plant sciences, and assessments of the performance of plant biotechnology products in practical applications.