Antimicrobial efficacy of Phoenix dactylifera-Infused three-dimensional-printed provisional dental resin against Porphyromonas gingivalis, Candida albicans, and Staphylococcus aureus: An in vitro study.
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引用次数: 0
Abstract
Aim: This study aimed to evaluate the antimicrobial efficacy and cytocompatibility of methanolic Phoenix dactylifera seed extract incorporated into three-dimensional (3D)-printed provisional dental resin against Porphyromonas gingivalis, Candida albicans, and Staphylococcusaureus.
Settings and design: This study design involves an in vitro experimental study.
Materials and methods: Methanolic extract of P. dactylifera seed was prepared and incorporated into a 3D-printed provisional dental resin at concentrations of 1.5%, 2.5%, and 5%. Antimicrobial activity was assessed by colony-forming unit (CFU/ml) counts for each microorganism. Cytotoxicity was evaluated using the MTT assay on Vero cells after 48 h. Phytochemical screening was performed to identify the bioactive constituents in the extract.
Statistical analysis used: One-way Analysis of Variance followed by Tukey's post hoc test was performed using IBM SPSS Statistics for Windows, Version 23.0.
Results: Phytochemical analysis revealed reducing sugars, alkaloids, flavonoids, tannins, glycosides, proteins, and resins. Antimicrobial efficacy increased with concentration, with the 5% group showing the most significant reduction in CFU/ml across all tested microorganisms (C. albicans: 0.13; P. gingivalis and S. aureus: P < 0.05). Cytotoxicity was minimal at 5% and 10% (cell viability >92%), while moderate cytotoxicity was observed at 25% (cell viability: 74%).
Conclusion: Incorporation of P. dactylifera seed extract into 3D-printed provisional dental resin enhanced antimicrobial activity against S. aureus, P. gingivalis, and C. albicans in a dose-dependent manner, with the 5% concentration showing the highest efficacy. Lower concentrations (1.5% and 2.5%) provided substantial antimicrobial effects while maintaining high biocompatibility, supporting their potential clinical use.
目的：研究三维打印临时牙树脂中甲醇凤尾籽提取物对牙龈卟啉单胞菌、白色念珠菌和金黄色葡萄球菌的抑菌效果和细胞相容性。设置和设计：本研究设计包括体外实验研究。材料与方法：制备羊蹄草种子甲醇提取物，分别以1.5%、2.5%、5%的浓度加入3d打印临时牙科树脂中。通过菌落形成单位（CFU/ml）计数来评估每种微生物的抗菌活性。48小时后，使用MTT法对Vero细胞进行细胞毒性评估。进行植物化学筛选以鉴定提取物中的生物活性成分。采用的统计分析：采用IBM SPSS Statistics for Windows， Version 23.0进行单向方差分析和Tukey事后检验。结果：植物化学分析发现了还原糖、生物碱、黄酮类化合物、单宁、糖苷、蛋白质和树脂。抗菌效果随浓度增加而增加，5%组的CFU/ml降低幅度最大（白色念珠菌：0.13；牙龈假单胞菌和金黄色葡萄球菌：P < 0.05）。细胞毒性在5%和10%时最小（细胞活力为92%），而在25%时观察到中度细胞毒性（细胞活力为74%）。结论：在3d打印临时牙树脂中加入短叶草种子提取物，可增强其对金黄色葡萄球菌、牙龈假单胞菌和白色假单胞菌的抑菌活性，且呈剂量依赖性，以5%的浓度效果最佳。较低浓度（1.5%和2.5%）在保持高生物相容性的同时提供了显著的抗菌效果，支持其潜在的临床应用。
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