The Effect of Light Intensity and Spectral Composition on Electron Transport in Chloroplasts in situ and in silico

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology

Biophysics Pub Date : 2025-10-20 DOI:10.1134/S0006350925700502

B. V. Trubitsin, A. V. Vershubskii, D. T. Guseinova, A. N. Tikhonov

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Abstract

Light-induced redox transformations of P₇₀₀, the reaction center of photosystem I, were studied using the EPR method depending on the illumination conditions of plant leaves (intensity and spectral composition of the active light). Within the framework of a mathematical model, the key stages of electron transfer along the noncyclic electron transport chain containing photosystems I and II and mobile transporters (plastoquinone, plastocyanin, and ferredoxin) and the processes of trans-thylakoid proton transfer associated with ATP synthesis were considered. The mechanisms of pH-dependent regulation of electron transport in chloroplasts at the acceptor and donor sites of photosystem I have been analyzed. The modeling results are in agreement with experimental data on the kinetics of light-induced transformations of P₇₀₀ in chloroplasts of higher plants. The results are discussed in the context of “short-term” pH-dependent mechanisms of electron transport regulation in chloroplasts in situ.

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光强和光谱组成对叶绿体原位和硅态电子传递的影响

利用EPR法研究了光系统I反应中心P700在植物叶片光照条件下（主动式光强度和光谱组成）的光诱导氧化还原转化。在数学模型的框架内，考虑了包含光系统I和II和移动转运体（质体醌、质体青素和铁氧化还蛋白）的非循环电子传递链上的电子转移的关键阶段，以及与ATP合成相关的反式类囊体质子转移过程。本文分析了光系统I受体和供体叶绿体中电子传递的ph依赖性调控机制。模拟结果与高等植物叶绿体中P700光诱导转化动力学的实验数据一致。这些结果是在“短期”的背景下讨论ph依赖的电子传输调节机制在叶绿体原位。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.