Enhancing Wolffia globosa cultivation: Feasibility of aerated suspended-growth systems as alternative cultivating option and the influence of abiotic factors on biomass production, CO₂ capture, and biochemical composition

IF 4.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-15 DOI:10.1016/j.procbio.2025.10.006

Kittikoon Sucunthowong , Shiva Rezaei Motlagh , Jareeluk Plengsakul , Sorawit Powtongsook , Kasidit Nootong

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Abstract

This study evaluated the feasibility of well-mixed suspended-growth systems for cultivating Wolffia globosa and investigated the effects of abiotic factors on growth, CO₂ capture, and biochemical composition of Wolffia. Continuous aeration at 0.4 and 0.8 vvm significantly improved biomass productivity compared to static water cultivation systems, while continuous stirring at 600 rpm caused extensive frond damage and lower biomass concentrations. Under optimized aerated conditions (500 mg/L initial biomass, 80 mg N/L initial nitrate, and 402 μmol photon/m²·s light intensity), the maximum biomass productivity reached 199.94 ± 25.88 mg/L·d, representing 83–277 % increase over static water cultivation. The corresponding CO₂ fixation rate (283.71 ± 36.73 mg CO₂/L·d) was comparable to those of Chlorella, highlighting the potential of W. globosa for biological CO₂ mitigation. Protein contents under optimized condition was 21.5 % of dry weight, with nitrate availability positively influencing protein accumulation. Key carotenoids, including lutein, β-carotene, neoxanthin, and violaxanthin, were detected, with no significant difference (p > 0.05) between suspended growth and static water systems. Furthermore, due to large frond size, biomass harvesting can be effectively conducted using filtration with low-cost, locally available materials.

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加强全球沃尔夫的培养：曝气悬浮生长系统作为替代培养方案的可行性以及非生物因素对生物量生产、CO 2捕获和生化组成的影响

本研究评估了混合悬浮体系培养全球狼尾草的可行性，并研究了非生物因素对狼尾草生长、CO2捕获和生化组成的影响。与静态水培养系统相比，0.4和0.8 vvm的连续曝气显著提高了生物量生产力，而600 rpm的连续搅拌造成了广泛的叶片损伤和较低的生物量浓度。在初始生物量500 mg/L、初始硝酸盐80 mg N/L、光照强度402 μmol光子/m2·s的优化条件下，最大生物量生产力达到199.94 ± 25.88 mg/L·d，比静态水栽培提高83 ~ 277 %。相应的CO2固定率（283.71 ± 36.73 mg CO2/L·d）与小球藻相当，凸显了小球藻在生物CO2减缓方面的潜力。优化条件下蛋白质含量为干重的21.5 %，硝态氮有效性对蛋白质积累有正向影响。关键类胡萝卜素，包括叶黄素、β-胡萝卜素、新黄质和紫黄质，在悬浮生长和静态水系统之间无显著差异（p >； 0.05）。此外，由于大叶尺寸，生物质收集可以有效地进行过滤与低成本，当地可用的材料。

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来源期刊

Process Biochemistry 生物-工程：化工

CiteScore

8.30

自引率

4.50%

发文量

374

审稿时长

53 days

期刊介绍： Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.