Transcriptomic and physiological analyses revealed nicotianamine enhances wheat tolerance to excess manganese

IF 4.1 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience Pub Date : 2025-10-03 DOI:10.1016/j.isci.2025.113671

Daozhen Luo (罗道祯) , Qing Li (李青) , Fei Pang (庞妃) , Wenjie Zhang (张文洁) , Muhammad Usman , Yangrui Li (李杨瑞) , Yongxiu Xing (邢永秀) , Dengfeng Dong (董登峰)

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Abstract

Manganese (Mn) is an essential plant micronutrient but toxic at supra-optimal concentrations. Although nicotianamine (NA) biosynthesis is metal inducible, its specific role in Mn detoxification in wheat remains poorly characterized. Integrated transcriptomic and physiological analyses of Mn-tolerant (ET8, Carazinho) and Mn-sensitive (ES8, Egret) wheat near-isogenic lines and their parental cultivars under excess Mn revealed pronounced upregulation of genes encoding nicotianamine synthase (TaNAS), nicotianamine aminotransferase (TaNAAT), and putative Mn-NA transporters (TaYSL2, TaYSL6) in tolerant genotypes. This transcriptional response correlated with elevated NA accumulation in roots under Mn stress. Exogenous NA application enhanced Mn tolerance in hydroponically grown seedlings in a concentration-dependent manner, accompanied by reduced tissue Mn accumulation, and maintained homeostasis of essential cations (Ca, Mg, Fe, Zn, Cu) in both roots and shoots. Our findings demonstrate that NA biosynthesis and transport contribute to Mn tolerance by modulating Mn partitioning and metal ion homeostasis. We further propose candidate genes (TaNAS, TaNAAT, TaYSL2, TaYSL6) as potential targets for breeding Mn-tolerant wheat.

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转录组学和生理学分析表明，烟胺增强了小麦对过量锰的耐受性

锰（Mn）是一种必需的植物微量营养素，但在超理想浓度下是有毒的。虽然烟胺（NA）的生物合成是金属诱导的，但其在小麦锰解毒中的具体作用仍不清楚。对Mn耐受型（ET8、Carazinho）和Mn敏感型（ES8、Egret）小麦近等基因系及其亲本进行的综合转录组学和生理分析显示，Mn耐受基因型中编码烟胺合成酶（TaNAS）、烟胺氨基转移酶（TaNAAT）和推测的Mn- na转运蛋白（TaYSL2、TaYSL6）的基因显著上调。这种转录反应与锰胁迫下根系NA积累增加有关。外源NA以浓度依赖的方式增强了水培幼苗的Mn耐受性，同时减少了组织Mn积累，并维持了根和芽中必需阳离子（Ca, Mg, Fe, Zn, Cu）的稳态。我们的研究结果表明，NA的生物合成和运输通过调节Mn分配和金属离子稳态来促进Mn耐受性。我们进一步提出候选基因（TaNAS, TaNAAT, TaYSL2, TaYSL6）作为选育耐锰小麦的潜在靶点。

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

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