Comparative evaluation of blood component preparation with the top-and-top and top-and-bottom methods: A change in Uruguay.

IF 1.4 4区医学 Q3 HEMATOLOGY

Transfusion Medicine Pub Date : 2025-10-18 DOI:10.1111/tme.70039

Natalia Méndez Acosta, Gabriela Rivas Alén, Ismael Rodriguez Grecco, Allexandra Díaz, Dalia Moreno, Ludwig R Frontier Ramos

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引用次数: 0

Abstract

Background: Hospital de Clínicas, in conjunction with the Universidad de la República UDELAR in Uruguay, evaluated the quality of blood components after separation with a top-and-bottom (TB) system, comparing it with the top-and-top (TT) system and verifying compliance with local and international standards.

Study design and methods: Whole blood (WB) was collected using TB (n = 150) and TT (n = 130). TB units were processed with MacoPress Smart, obtaining red cell concentrate (RCC), plasma (PL), and buffy coat (BC). After 2 h, BC had a soft spin centrifugation for a single unit of platelet concentrate (SUPC). TT bags were managed on day 0 (up to 8 h of collection) with manual press obtaining RCC and platelet-rich plasma units (PRP). PRP had hard spin centrifugation and was separated again with the manual press to collect PL and SUPC.

Results: TB system produced RCC with higher haemoglobin concentration and Haematocrit but with lower volume and Hb/unit (p < 0.001). The TB system produced RCC with lower leukocytes and residual platelet levels (p < 0.001). SUPC showed fewer residual leukocytes and red cell levels with TB (p < 0.001), but TT produced a higher mean platelet count than the TB system (p < 0.001). During this evaluation, there was no significant difference in the mean plasma volume (p = 0.178).

Conclusion: The analysis favors the implementation of TB in the HC Blood Bank, improving the quality and safety of the transfusion service.

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顶顶法和顶底法制备血液成分的比较评价：乌拉圭的变化。

背景：Clínicas医院与乌拉圭República UDELAR大学合作，评估了采用顶底（TB）系统分离后血液成分的质量，将其与顶顶（TT）系统进行比较，并验证其是否符合当地和国际标准。研究设计与方法：采用TB （n = 150）和TT （n = 130）采集全血（WB）。结核细胞用MacoPress Smart处理，获得红细胞浓缩物（RCC）、血浆（PL）和灰白色被膜（BC）。2小时后，BC对单个血小板浓缩物（SUPC）进行软自旋离心。TT袋在第0天（收集至8小时）用手动按压获得RCC和富血小板血浆单位（PRP）。PRP进行硬旋离心，再用手动压机分离，收集PL和SUPC。结果：TB系统产生的RCC血红蛋白浓度和红细胞压积较高，但体积和Hb/单位较低(p)。结论：该分析有利于HC血库实施TB，提高输血服务的质量和安全性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Transfusion Medicine 医学-血液学

CiteScore

2.70

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Transfusion Medicine publishes articles on transfusion medicine in its widest context, including blood transfusion practice (blood procurement, pharmaceutical, clinical, scientific, computing and documentary aspects), immunohaematology, immunogenetics, histocompatibility, medico-legal applications, and related molecular biology and biotechnology. In addition to original articles, which may include brief communications and case reports, the journal contains a regular educational section (based on invited reviews and state-of-the-art reports), technical section (including quality assurance and current practice guidelines), leading articles, letters to the editor, occasional historical articles and signed book reviews. Some lectures from Society meetings that are likely to be of general interest to readers of the Journal may be published at the discretion of the Editor and subject to the availability of space in the Journal.