{"title":"Vacuolar transport and function of Saccharomyces cerevisiae sterol ester hydrolase Tgl1.","authors":"Takumi Nakatsuji, Kosuke Shiraishi, Chuqian Wang, Hiroya Yurimoto, Yasuyoshi Sakai, Masahide Oku","doi":"10.1002/1873-3468.70196","DOIUrl":null,"url":null,"abstract":"<p><p>Sterol ester hydrolases (SEHs) play an important role in the quantitative regulation of sterols. Mammalian cells are known to possess SEHs both on the surface of lipid droplets and inside lysosomes. However, to date, no studies on the yeast Saccharomyces cerevisiae have identified active SEHs in the vacuole, which is the corresponding organelle to the mammalian lysosome. Here, we show that S. cerevisiae Tgl1 functions as the major SEH in the vacuole after being transported into the organelle lumen, in addition to its role in the cytoplasm. The transport of Tgl1 into the vacuole was independent of macroautophagy and ESCRT (endosomal sorting complex required for transport) complex-0 component Vps27 but dependent on ESCRT-I-III components. This study also revealed the mechanism of formation of vacuolar membrane microdomains supported by the SEHs.</p>","PeriodicalId":12142,"journal":{"name":"FEBS Letters","volume":" ","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2025-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"FEBS Letters","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1002/1873-3468.70196","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Sterol ester hydrolases (SEHs) play an important role in the quantitative regulation of sterols. Mammalian cells are known to possess SEHs both on the surface of lipid droplets and inside lysosomes. However, to date, no studies on the yeast Saccharomyces cerevisiae have identified active SEHs in the vacuole, which is the corresponding organelle to the mammalian lysosome. Here, we show that S. cerevisiae Tgl1 functions as the major SEH in the vacuole after being transported into the organelle lumen, in addition to its role in the cytoplasm. The transport of Tgl1 into the vacuole was independent of macroautophagy and ESCRT (endosomal sorting complex required for transport) complex-0 component Vps27 but dependent on ESCRT-I-III components. This study also revealed the mechanism of formation of vacuolar membrane microdomains supported by the SEHs.
甾醇酯水解酶（SEHs）在甾醇的定量调控中起着重要作用。已知哺乳动物细胞在脂滴表面和溶酶体内部都具有SEHs。然而，到目前为止，还没有关于酵母酿酒酵母的研究发现液泡中有活性SEHs，液泡是哺乳动物溶酶体的相应细胞器。在这里，我们发现酿酒酵母Tgl1除了在细胞质中发挥作用外，在被运输到细胞器管腔后还在液泡中起主要的SEH作用。Tgl1转运到液泡中不依赖于巨噬和ESCRT（运输所需的内体分选复合物）复合物-0组分Vps27，但依赖于ESCRT- i - iii组分。该研究还揭示了由SEHs支持的液泡膜微域的形成机制。
期刊介绍：
FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.
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