Unraveling the molecular mechanisms of cadmium-induced stress in Mytilus galloprovincialis: Chaperone proteins as key mediators

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology Pub Date : 2025-10-15 DOI:10.1016/j.etap.2025.104847

Khouloud Boukadida , Mohamed Banni , Tiziana Cappello , Marouane Chemek , Imed Messaoudi , Hamadi Boussetta

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引用次数: 0

Abstract

Coastal ecosystems are highly exposed to cadmium (Cd), a widespread heavy metal with multifaceted toxicity. This study investigates the early cellular responses of Mytilus galloprovincialis exposed to two hazardous Cd concentrations (0.1 and 1 µM) for 4 days. Digestive gland analyses revealed a dose-dependent modulation of key stress biomarkers. Gene expression was quantified for proteins involved in folding (HSP90, HSP70, HSP27, HSP26, calreticulin, FKBP) and metal detoxification (metallothionein-10 and −20; MT-10, MT-20). Oxidative stress responses included catalase (CAT) and glutathione S-transferase (GST) activities, malondialdehyde (MDA) levels, and protein sulfhydryls (PSH), while MT levels were also measured. The results show a coordinated upregulation of chaperones and antioxidant defenses, reflecting an adaptive strategy to Cd-induced stress. Although these biomarkers are not specific to cadmium, their integrated responses provide mechanistic insight into early cellular perturbations, highlighting their potential value for biomarker-based environmental monitoring in coastal ecosystems.

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镉胁迫对紫贻贝（Mytilus galloprovincialis）分子机制的影响：伴侣蛋白作为关键介质。

沿海生态系统高度暴露于镉（Cd），这是一种广泛存在的具有多方面毒性的重金属。本研究研究了在两种有害Cd浓度（0.1µM和1µM）下暴露4天的紫贻贝（Mytilus galloprovincialis）的早期细胞反应。消化腺分析揭示了关键应激生物标志物的剂量依赖性调节。对参与折叠蛋白（HSP90、HSP70、HSP27、HSP26、calreticulin、FKBP）和金属解毒蛋白（metallothionein-10和-20；MT-10、MT-20）的基因表达进行定量分析。氧化应激反应包括过氧化氢酶（CAT）和谷胱甘肽s -转移酶（GST）活性、丙二醛（MDA）水平和蛋白巯基（PSH）水平，MT水平也被测量。结果显示了伴侣蛋白和抗氧化防御的协调上调，反映了对cd诱导的应激的适应性策略。虽然这些生物标志物不是镉特异性的，但它们的综合反应提供了对早期细胞扰动的机制洞察，突出了它们在沿海生态系统中基于生物标志物的环境监测中的潜在价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Environmental toxicology and pharmacology 医学-毒理学

CiteScore

7.00

自引率

4.70%

发文量

185

审稿时长

34 days

期刊介绍： Environmental Toxicology and Pharmacology publishes the results of studies concerning toxic and pharmacological effects of (human and veterinary) drugs and of environmental contaminants in animals and man. Areas of special interest are: molecular mechanisms of toxicity, biotransformation and toxicokinetics (including toxicokinetic modelling), molecular, biochemical and physiological mechanisms explaining differences in sensitivity between species and individuals, the characterisation of pathophysiological models and mechanisms involved in the development of effects and the identification of biological markers that can be used to study exposure and effects in man and animals. In addition to full length papers, short communications, full-length reviews and mini-reviews, Environmental Toxicology and Pharmacology will publish in depth assessments of special problem areas. The latter publications may exceed the length of a full length paper three to fourfold. A basic requirement is that the assessments are made under the auspices of international groups of leading experts in the fields concerned. The information examined may either consist of data that were already published, or of new data that were obtained within the framework of collaborative research programmes. Provision is also made for the acceptance of minireviews on (classes of) compounds, toxicities or mechanisms, debating recent advances in rapidly developing fields that fall within the scope of the journal.