Ultrastructural characterization of neuropeptide Y synapses in the central inferior colliculus of the Fischer Brown Norway rat.

IF 2.7 3区医学 Q3 ENDOCRINOLOGY & METABOLISM

Neuropeptides Pub Date : 2025-10-13 DOI:10.1016/j.npep.2025.102566

Laila S Almassri, Joshua C Harris, Kristen M Crane, Andrew P Ohl, Nick J Tokar, Brett R Schofield, Jesse W Young, Jeffrey G Mellott

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引用次数: 0

Abstract

Introduction: The central nucleus of the inferior colliculus (ICc) is the primary target for ascending auditory projections from the lower auditory brainstem nuclei and the main source of ascending projections to the auditory thalamus. The ICc has an extensive network of tonotopically organized neurons, whose classification is a subject of broad interest. In order to understand the extensive microcircuitry of the ICc, it is important to ascertain the synaptic arrangements among molecularly identified neuronal populations. The objective of this study was to describe inhibitory Neuropeptide Y (NPY) synapses in the central nucleus of the IC.

Method: We used electron microscopy (EM) and post-embedding anti-NPY immunogold histochemistry on tissue from adult Fischer Brown Norway rats. Inhibitory NPY synapses were identified by pleomorphic vesicles, symmetric synaptic junctions, and NPY-immunopositive presynaptic boutons.

Results: The data demonstrate that NPY terminals largely (∼75 %) form symmetric synapses with pleomorphic vesicles, mostly target small and medium dendrites, and have larger average bouton areas, active zone lengths, and vesicle pools, as compared to the overall GABAergic population.

Discussion: The subset of GABAergic neurons that co-express NPY feature synaptic characteristics suggesting that NPY neuromodulation has a widespread role in regulating excitation in the ICc.

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Fischer Brown Norway大鼠中央下丘神经肽Y突触的超微结构表征。

下丘中央核是来自下听脑干核的上行听觉投射的主要目标，也是听觉丘脑上行投射的主要来源。ICc有一个广泛的拓扑组织神经元网络，其分类是一个广泛感兴趣的主题。为了了解ICc的广泛微电路，确定分子识别的神经元群体之间的突触排列是很重要的。方法：采用电镜和免疫组化方法对成年褐威大鼠组织进行抗神经肽Y （NPY）包埋后免疫组化。抑制性NPY突触通过多形性囊泡、对称突触连接和NPY免疫阳性的突触前钮扣来鉴定。结果：数据表明，NPY末端大部分（~ 75%）形成具有多形性囊泡的对称突触，主要针对中小型树突，并且与总体gaba能群体相比，具有更大的平均钮扣面积，活性区长度和囊泡池。讨论：共同表达NPY的gaba能神经元子集具有突触特征，这表明NPY神经调节在ICc的兴奋调节中具有广泛的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neuropeptides 医学-内分泌学与代谢

CiteScore

5.40

自引率

6.90%

发文量

审稿时长

>12 weeks

期刊介绍： The aim of Neuropeptides is the rapid publication of original research and review articles, dealing with the structure, distribution, actions and functions of peptides in the central and peripheral nervous systems. The explosion of research activity in this field has led to the identification of numerous naturally occurring endogenous peptides which act as neurotransmitters, neuromodulators, or trophic factors, to mediate nervous system functions. Increasing numbers of non-peptide ligands of neuropeptide receptors have been developed, which act as agonists or antagonists in peptidergic systems. The journal provides a unique opportunity of integrating the many disciplines involved in all neuropeptide research. The journal publishes articles on all aspects of the neuropeptide field, with particular emphasis on gene regulation of peptide expression, peptide receptor subtypes, transgenic and knockout mice with mutations in genes for neuropeptides and peptide receptors, neuroanatomy, physiology, behaviour, neurotrophic factors, preclinical drug evaluation, clinical studies, and clinical trials.