Ultrasound-responsive hybrid liposome-polymer nanoparticles for enhanced stability and cytotoxicity of chemotherapeutic agent.

IF 3.1 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2025-11-01 Epub Date: 2025-10-17 DOI:10.1080/02652048.2025.2570650

Sidrah Saeed, Usama Sarwar, Imran Tariq, Afreenish Amir, Abida Raza

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引用次数: 0

Abstract

Aims: To develop and evaluate a biocompatible, stable, ultrasound-responsive hybrid nanocarrier for effective therapeutic outcomes.

Methods: The hybrid liposome-polymer drug delivery system (hLPDS) was characterised using dynamic light scattering, photoluminescence, atomic force microscopy, Raman spectroscopy and evaluated for drug release, cytotoxicity, cell uptake, and biodistribution.

Results: The hLPDS demonstrated mean diameter of 120 ± 5 nm and polydispersity index of 0.58 ± 0.83, zeta potential of 30 ± 5 mV, encapsulation efficiency of 93 ± 2.9%, loading capacity of 13 ± 1.5% (w/w) and remained stable for 90 days. The hLPDS showed 10% cell viability and lower IC₅₀ (1.274 ± 0.32 µM) compared to free drug (1.842 ± 0.32 µM). Ultrasound at 4 MHz frequency increased drug release above 90% (w/v), enhanced cellular internalisation (p < 0.0001), and anti-proliferative effects.

Conclusion: Ultrasound-triggered hLPDS exhibited enhanced cytotoxicity, cell uptake, stability, and therapeutic effect compared to liposomes and micelles alone.

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超声响应的混合脂质体-聚合物纳米颗粒增强化疗药物的稳定性和细胞毒性。

目的：开发和评估一种生物相容性、稳定性、超声响应的混合纳米载体，以获得有效的治疗效果。方法：采用动态光散射、光致发光、原子力显微镜、拉曼光谱等方法对脂质体-聚合物混合给药体系（hLPDS）进行表征，并对其药物释放、细胞毒性、细胞摄取和生物分布进行评价。结果：hLPDS的平均直径为120±5 nm，多分散性指数为0.58±0.83，zeta电位为30±5 mV，包封效率为93±2.9%，载药量为13±1.5% (w/w)，稳定性为90 d。hLPDS的细胞存活率为10%，IC50（1.274±0.32µM）低于游离药物（1.842±0.32µM）。结论：超声触发的hLPDS与单独使用脂质体和胶束相比，具有更强的细胞毒性、细胞摄取、稳定性和治疗效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.