Structure-function relationship of the GH168 fucanase reveals an unusual enzyme recognition mechanism for sulfated polysaccharide.

IF 15.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Nature Communications Pub Date : 2025-10-17 DOI:10.1038/s41467-025-63891-4

Guangning Chen,Jingjing Shen,Xinyu Li,Menghui Sun,Sheng Dong,Ying Sun,Achiraya Khamleng,Xuanwei Mei,Yuying Zhang,Guanchen Liu,Fangyi Chen,Xiao Song,Kaimeng Liu,Yingang Feng,Changhu Xue,Yaoguang Chang

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引用次数: 0

Abstract

Sulfated fucan is one of the most recalcitrant polysaccharides. The molecular mechanism underlying the endo-1,3-fucanase, which plays a critical role in the breakdown of sulfated fucan, remains unexplained. Here, we conduct a comprehensive structure-function relationship investigation on the endo-1,3-fucanases within a family space-GH168. The family can be divided into four subfamilies according to phylogenetic relationship and functional similarities. Subfamily I, Ⅱ and Ⅳ preferentially recognize Fucp2(OSO3-), Fucp2,4(OSO3-) and Fucp units at the +1 subsite, respectively, while consistently recognizing the Fucp2(OSO3-) unit at the -1 subsite. Remarkably, two-thirds of the interacting residues are dedicated to the recognition of sulfate groups along the glycoside chains. This mechanism is distinct from the direct recognition of the sugar backbone employed by neutral polysaccharide hydrolases. These findings unveil a critical enzyme recognition mechanism for sulfate polysaccharides and promote the application of endo-1,3-fucanases in the structural analysis and oligosaccharide production of sulfated fucan.

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GH168岩藻聚糖酶的结构-功能关系揭示了一种不同寻常的硫酸盐多糖酶识别机制。

硫酸酸化岩藻多糖是最顽固的多糖之一。内切-1,3-岩藻聚糖酶在酸化岩藻聚糖的分解中起着关键作用，其分子机制尚不清楚。在此，我们对家族空间- gh168中的内切-1,3-岩藻聚糖酶进行了全面的结构-功能关系研究。根据系统发育关系和功能相似性，该科可分为4个亚科。亚家族I、Ⅱ和Ⅳ分别优先识别+1亚位点上的Fucp2（OSO3-）、Fucp2、4（OSO3-）和Fucp单元，同时一致识别-1亚位点上的Fucp2（OSO3-）单元。值得注意的是，三分之二的相互作用残基专门用于识别沿糖苷链的硫酸盐基团。这种机制不同于中性多糖水解酶直接识别糖骨架。这些发现揭示了硫酸盐多糖的关键酶识别机制，并促进了内切-1,3-岩藻聚糖酶在酸化岩藻聚糖结构分析和低聚糖生产中的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Communications Biological Science Disciplines-

CiteScore

24.90

自引率

2.40%

发文量

6928

审稿时长

3.7 months

期刊介绍： Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.