Synthesis, characterization, and antifungal activity of metallic nanoparticles and simvastatin against fungi that cause superficial infections

IF 2.4 4区医学 Q3 MYCOLOGY

Journal de mycologie medicale Pub Date : 2025-12-01 Epub Date: 2025-09-09 DOI:10.1016/j.mycmed.2025.101580

Diego Henrique Morandi , Maria Gabriela Martins dos Reis , Aline Ratuchne , Ricardo Sérgio Couto de Almeida , Guilherme Fonseca Reis , Luciano Aparecido Panagio

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引用次数: 0

Abstract

Superficial mycoses present several clinical manifestations, ranging from lesions of the skin and its appendages to progression into disseminated infections. Antifungal resistance to currently available drugs is a global public health concern, necessitating novel antimicrobial compounds. Metal nanoparticles or simvastatin (SIM), via drug repositioning, represent promising avenues. The aims of this study were to synthesize and characterize silver nanoparticles (AgNPs) and selenium nanoparticles (SeNPs) and to evaluate their antifungal activity in combination with simvastatin against T. mentagrophytes, T. rubrum, N. gypsea, C. albicans, C. tropicalis, and M. pachydermatis. Nanoparticles were biosynthesized using the supernatant of L. ornata and characterized by UV–Vis, FTIR, zeta potential analysis, DLS, and EDXRF. Their antifungal activity was assessed via broth microdilution (MIC) and checkerboard assays to evaluate agent interactions. MIC values ranged from 1 to 8.7 µg/mL for AgNPs, 194.5 to 778 µg/mL for SeNPs, and 78 to 312.5 µg/mL for simvastatin. A synergistic effect was observed between AgNPs and simvastatin against T. rubrum and T. mentagrophytes. The L. ornata strain demonstrated the ability to synthesize AgNPs and SeNPs, though further research is required to optimize SeNP production. The tested compounds displayed antifungal activity against most of the species analyzed, highlighting their potential as promising alternatives for treating superficial mycoses and combating antifungal resistance.

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金属纳米颗粒和辛伐他汀对引起表面感染的真菌的合成、表征和抗真菌活性。

浅表真菌病有几种临床表现，从皮肤及其附属物的损伤到进展为播散性感染。对现有药物的抗真菌耐药性是一个全球性的公共卫生问题，需要新的抗菌化合物。通过药物重新定位，金属纳米颗粒或辛伐他汀（SIM）代表了有希望的途径。本研究的目的是合成纳米银粒子（AgNPs）和纳米硒粒子（SeNPs）并对其进行表征，并评价其与辛伐他汀联合使用对植物T. mentagrophytes、T. rubrum、N. gypsea、C. albicans、C. tropicalis和厚皮分枝杆菌的抗真菌活性。利用鸢尾上清液合成纳米颗粒，并通过紫外可见光谱、红外光谱、zeta电位分析、DLS和EDXRF对其进行了表征。通过微量肉汤稀释法（MIC）和棋盘法（棋盘法）评估其抗真菌活性。AgNPs的MIC值为1 ~ 8.7µg/mL， SeNPs的MIC值为194.5 ~ 778µg/mL，辛伐他汀的MIC值为78 ~ 312.5µg/mL。AgNPs与辛伐他汀对红藓和红藓有协同作用。L. ornata菌株显示出合成AgNPs和SeNPs的能力，但需要进一步研究优化SeNP的生产。测试的化合物对大多数分析的物种显示出抗真菌活性，突出了它们作为治疗浅表真菌病和对抗抗真菌耐药性的有希望的替代品的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal de mycologie medicale 医学-真菌学

CiteScore

5.10

自引率

2.80%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal de Mycologie Medicale / Journal of Medical Mycology (JMM) publishes in English works dealing with human and animal mycology. The subjects treated are focused in particular on clinical, diagnostic, epidemiological, immunological, medical, pathological, preventive or therapeutic aspects of mycoses. Also covered are basic aspects linked primarily with morphology (electronic and photonic microscopy), physiology, biochemistry, cellular and molecular biology, immunochemistry, genetics, taxonomy or phylogeny of pathogenic or opportunistic fungi and actinomycetes in humans or animals. Studies of natural products showing inhibitory activity against pathogenic fungi cannot be considered without chemical characterization and identification of the compounds responsible for the inhibitory activity. JMM publishes (guest) editorials, original articles, reviews (and minireviews), case reports, technical notes, letters to the editor and information. Only clinical cases with real originality (new species, new clinical present action, new geographical localization, etc.), and fully documented (identification methods, results, etc.), will be considered. Under no circumstances does the journal guarantee publication before the editorial board makes its final decision. The journal is indexed in the main international databases and is accessible worldwide through the ScienceDirect and ClinicalKey platforms.