Electrochemical biosensor for CA125 detection based on ZIF-8@Au and TME-PTMC dual signal amplification

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-10-17 DOI:10.1007/s00604-025-07548-5

Chong Li, Fei Zhang, Xia Wang, Qi Xue, Zhe Qin, Hualu Jia, Huaixia Yang

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Abstract

Cancer antigen 125 (CA125) is a key biomarker for ovarian cancer, and its concentration level is an important predictor for diagnosis. We developed a novel electrochemical impedance biosensor for CA125 detection based on a dual-signal amplification strategy using 1,1,1-tris(hydroxymethyl)ethane-polytrimethylene carbonate (TME-PTMC) and zeolitic imidazolate framework-8 loaded with gold nanoparticles (ZIF-8@Au). The biosensor was constructed by immobilizing ZIF-8@Au on a gold electrode using 1,6-hexanedithiol (HDT) as a crosslinking agent, followed by the attachment of aptamer 1 (Apt1) via sulfhydryl groups. 6-Mercapto-1-hexanol (MCH) was used to prevent nonspecific binding. Through specific aptamer-antigen recognition, CA125 was captured and anchored onto the electrode surface. After carbodiimide hydrochloride (EDC)/ N-hydroxysuccinimide (NHS) activation, aptamer 2 (Apt2) further recognized CA125, forming an aptamer-antigen-aptamer "sandwich structure". The TME-PTMC polymer was then conjugated via ester bonds, significantly enhancing signal amplification. Electrochemical impedance spectroscopy (EIS) analysis confirmed that under optimized conditions, the sensor exhibited a wide linear detection range (0.01 U mL⁻¹ to 100 U mL⁻¹) and a low detection limit (0.0062 U mL⁻¹). Moreover, the sensor demonstrated excellent selectivity, stability, and reproducibility in the analysis of clinical serum samples, highlighting its potential for early ovarian cancer diagnosis and clinical monitoring.

Graphical Abstract

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基于ZIF-8@Au和TME-PTMC双信号放大的CA125检测电化学生物传感器。

癌抗原125 （Cancer antigen 125, CA125）是卵巢癌的重要生物标志物，其浓度水平是诊断卵巢癌的重要指标。我们基于双信号放大策略，利用1,1,1-三（羟甲基）乙烷-聚三亚甲基碳酸酯（TME-PTMC）和负载金纳米颗粒的沸石咪唑酸骨架-8 （ZIF-8@Au）开发了一种新型的CA125检测电化学阻抗生物传感器。以1,6-己二硫醇（HDT）为交联剂，将ZIF-8@Au固定在金电极上，然后通过巯基连接适配体1 (Apt1)，构建生物传感器。6-巯基-1-己醇（MCH）用于防止非特异性结合。通过特异性适配体抗原识别，CA125被捕获并固定在电极表面。经盐酸碳二亚胺(EDC)/ n-羟基琥珀酰亚胺（NHS）活化后，适体2 （Apt2）进一步识别CA125，形成适体-抗原-适体“三明治结构”。然后通过酯键共轭TME-PTMC聚合物，显著增强信号放大。电化学阻抗谱（EIS）分析证实，在优化条件下，该传感器具有较宽的线性检测范围（0.01 U mL-1 ~ 100 U mL-1）和较低的检出限（0.0062 U mL-1）。此外，该传感器在临床血清样本分析中表现出优异的选择性、稳定性和可重复性，突出了其在卵巢癌早期诊断和临床监测中的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.