Authentication of Panax notoginseng with high-efficiency Proofman-LMTIA technology

IF 3.6 3区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Methods Pub Date : 2025-12-01 Epub Date: 2025-10-14 DOI:10.1016/j.ymeth.2025.10.005

Xiaodong Zhang , Caixia Li , Yongzhen Wang , Yue Cao , Xiaona He , Fugang Xiao , Deguo Wang

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Abstract

Panax notoginseng, a cornerstone of traditional Chinese medicine, is frequently subject to adulteration in commercial markets, compromising its therapeutic efficacy and safety. This study introduces a novel application of Proofman-LMTIA technology to authenticate P. notoginseng with high precision and efficiency. By targeting unique sequence variations in the ITS2 rDNA region, we developed species-specific primers and probe to distinguish P. notoginseng from common adulterants. The method achieves a detection sensitivity of 10 pg/µL and identifies adulteration at levels as low as 1 % (v/v), validated across diverse commercial products, including powders and capsules. With a detection time of under 30 min and no reliance on specialized equipment, this approach offers a streamlined, cost-efficient solution for quality assurance in the herbal industry. Our results demonstrate 100 % accuracy in market sample testing, addressing critical challenges in P. notoginseng authentication and supporting regulatory compliance.

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高效Proofman-LMTIA技术对三七的鉴别

三七是传统中药的基石，在商业市场上经常被掺假，影响其治疗功效和安全性。本研究介绍了一种新的proof - lmtia技术在三七鉴别中的应用，该技术具有高精度和高效率。利用ITS2 rDNA区域独特的序列变异，我们开发了物种特异性引物和探针来区分三七和常见的掺假物。该方法的检测灵敏度为10 pg/µL，可识别低至1 % （v/v）的掺假水平，并在各种商业产品（包括粉末和胶囊）中进行验证。检测时间在30 min以下，不依赖于专门的设备，这种方法为草药行业的质量保证提供了一种简化的、经济有效的解决方案。我们的研究结果证明了100% %的市场样品测试的准确性，解决了三七认证和支持法规遵从性的关键挑战。

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来源期刊

Methods 生物-生化研究方法

CiteScore

9.80

自引率

2.10%

发文量

222

审稿时长

11.3 weeks

期刊介绍： Methods focuses on rapidly developing techniques in the experimental biological and medical sciences. Each topical issue, organized by a guest editor who is an expert in the area covered, consists solely of invited quality articles by specialist authors, many of them reviews. Issues are devoted to specific technical approaches with emphasis on clear detailed descriptions of protocols that allow them to be reproduced easily. The background information provided enables researchers to understand the principles underlying the methods; other helpful sections include comparisons of alternative methods giving the advantages and disadvantages of particular methods, guidance on avoiding potential pitfalls, and suggestions for troubleshooting.