Elecsys CSF AD immunoassays: Sample stability for a new pre-analytical protocol for fresh CSF

IF 11.1 1区医学 Q1 CLINICAL NEUROLOGY

Alzheimer's & Dementia Pub Date : 2025-10-17 DOI:10.1002/alz.70797

Ekaterina Manuilova, Isabelle Schrurs, Sandra Rutz, Silja McIlwrick, Oliver Goldhardt, Patrick Sommer, Timo Grimmer

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引用次数: 0

Abstract

INTRODUCTION

Amyloid beta 1–42 (Aβ42), tau phosphorylated at threonine-181 (p-tau181), and total tau (t-tau) are cerebrospinal fluid (CSF) biomarkers for Alzheimer's disease diagnosis.

METHODS

We prospectively examined storage time, temperature, and freeze/thaw effects on Aβ42, p-tau181, and t-tau stability in fresh CSF samples using Elecsys^® CSF immunoassays.

RESULTS

All three biomarkers were stable at 2–8°C for ≤15 days, and −25°C to −15°C for ≤8 weeks, and after one freeze/thaw cycle. p-Tau181 and t-tau were also stable at 15–25°C for ≤8 days and at −25°C to −15°C for 12–15 weeks. Aβ42 recovery declined slightly after storage at 15–25°C for ≤8 days and −25°C to −15°C for 12–15 weeks, and one freeze/thaw cycle.

DISCUSSION

For optimal immunoassay performance, it is recommended to store CSF samples at 15–25°C for ≤5 days, 2–8°C for ≤15 days, and −25°C to −15°C for ≤8 weeks.

Highlights

Cerebrospinal fluid (CSF) biomarkers aid in Alzheimer's disease diagnosis.
Fresh CSF stored at 15–25°C for ≤5 days is optimal for Elecsys CSF immunoassays.
Fresh CSF stored at 2–8°C for ≤15 days is optimal for Elecsys CSF immunoassays.
Fresh CSF stored at −25°C to −15°C for ≤8 weeks is optimal for Elecsys CSF immunoassays.

Abstract Image

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Elecsys CSF AD免疫测定：新鲜CSF的新前分析方案的样品稳定性

淀粉样蛋白β 1-42 （Aβ42）、苏氨酸-181磷酸化的tau蛋白（p-tau181）和总tau蛋白（t-tau）是诊断阿尔茨海默病的脑脊液（CSF）生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Alzheimer's & Dementia 医学-临床神经学

CiteScore

14.50

自引率

5.00%

发文量

299

审稿时长

3 months

期刊介绍： Alzheimer's & Dementia is a peer-reviewed journal that aims to bridge knowledge gaps in dementia research by covering the entire spectrum, from basic science to clinical trials to social and behavioral investigations. It provides a platform for rapid communication of new findings and ideas, optimal translation of research into practical applications, increasing knowledge across diverse disciplines for early detection, diagnosis, and intervention, and identifying promising new research directions. In July 2008, Alzheimer's & Dementia was accepted for indexing by MEDLINE, recognizing its scientific merit and contribution to Alzheimer's research.