One-Week Elderberry Juice Intervention Promotes Metabolic Flexibility in the Transcriptome of Overweight Adults During a Meal Challenge.

IF 5 2区医学 Q1 NUTRITION & DIETETICS

Nutrients Pub Date : 2025-10-01 DOI:10.3390/nu17193142

Christy Teets, Andrea J Etter, Patrick M Solverson

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引用次数: 0

Abstract

Background: Metabolic flexibility, the ability to efficiently switch between fuel sources in response to changing nutrient availability and energy demands, is recognized as a key determinant of metabolic health. In a recent randomized controlled human feeding trial, overweight individuals receiving American black elderberry juice (EBJ) demonstrated improvements in multiple clinical indices of metabolic flexibility, but the mechanisms of action were unexplored. The objective of this study was to utilize RNA sequencing to examine how EBJ modulates the transcriptional response to fasting and feeding, focusing on pathways related to metabolic flexibility.

Methods: Overweight or obese adults (BMI > 25 kg/m²) without chronic illnesses were randomized to a 5-week crossover study protocol with two 1-week periods of twice-daily EBJ or placebo (PL) separated by a washout period. RNA sequencing was performed on peripheral blood mononuclear cells from 10 participants to assess transcriptomic responses collected at fasting (pre-meal) and postprandial (120 min post-meal) states during a meal-challenge test.

Results: The fasted-to-fed transition for EBJ showed 234 differentially expressed genes following EBJ consumption compared to 59 genes following PL, with 44 genes shared between interventions. EBJ supplementation showed significantly higher enrichment of several metabolic pathways including insulin, FoxO, and PI3K-Akt signaling. KEGG pathway analysis showed 27 significant pathways related to metabolic flexibility compared to 7 for PL.

Conclusions: Our findings indicate that short-term elderberry juice consumption may promote metabolic flexibility in overweight adults.

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一周接骨木汁干预促进超重成年人在膳食挑战期间转录组的代谢灵活性。

背景：代谢灵活性，即根据不断变化的营养可利用性和能量需求在燃料来源之间有效切换的能力，被认为是代谢健康的关键决定因素。在最近的一项随机对照人类喂养试验中，超重个体接受美国黑接骨木汁（EBJ）显示出代谢灵活性的多个临床指标的改善，但其作用机制尚不清楚。本研究的目的是利用RNA测序来研究EBJ如何调节禁食和喂养的转录反应，重点关注与代谢灵活性相关的途径。方法：无慢性疾病的超重或肥胖成人（BMI > 25 kg/m2）被随机分为5周的交叉研究方案，其中两个1周的时段每天两次EBJ或安慰剂（PL），中间间隔一个洗脱期。研究人员对10名参与者的外周血单个核细胞进行了RNA测序，以评估在进食刺激试验中空腹（餐前）和餐后（餐后120分钟）状态收集的转录组反应。结果：食用EBJ后，快速向进食的转变显示234个差异表达基因，而食用PL后，差异表达基因为59个，干预之间共有44个基因。补充EBJ可显著提高胰岛素、FoxO和PI3K-Akt信号通路的富集程度。KEGG通路分析显示27条与代谢柔韧性相关的重要通路，而pl7条。结论：我们的研究结果表明，短期饮用接骨木汁可能促进超重成年人的代谢柔韧性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nutrients NUTRITION & DIETETICS-

CiteScore

9.20

自引率

15.30%

发文量

4599

审稿时长

16.74 days

期刊介绍： Nutrients (ISSN 2072-6643) is an international, peer-reviewed open access advanced forum for studies related to Human Nutrition. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.