Steamed ginger extract ameliorates the symptoms of osteoarthritis in monosodium iodoacetate-induced osteoarthritis rats.

IF 2.1 4区医学 Q3 NUTRITION & DIETETICS

Nutrition Research and Practice Pub Date : 2025-10-01 Epub Date: 2025-04-14 DOI:10.4162/nrp.2025.19.5.682

Hyun Sook Lee, Jae In Jung, Seungtae Lim, Dae Won Park, Seong Ho Lee, Jaewoo Bae, Jae Kyoung Lee, Eun Ji Kim

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引用次数: 0

Abstract

Background/objectives: Steamed ginger extract (GGE03) has been proposed as a complementary treatment for osteoarthritis (OA). On the other hand, the efficacy and mechanism are incompletely understood. This study examined the anti-osteoarthritic effects of GGE03 in monosodium iodoacetate (MIA)-induced OA Sprague-Dawley (SD) rats.

Materials/methods: The rats were divided into the following groups: normal control group (NC); OA control group (OC); OA+30, 50, or 100 mg/kg body weight (BW)/day GGE03 group (O+G30, O+G50, or O+G100); OA+150 mg/kg BW/day methyl sulfonyl methane (MSM, positive control group (O+M). The SD rats were given GGE03 or MSM orally for 5 weeks. MIA was injected intra-articularly into the knee joints on day 15 to induce OA. The exercise performance test, pain behavior test, micro-computed tomography, histological examination, and immunofluorescence staining were performed on the knee joint. The inflammatory mediators, cytokines, and matrix metalloproteinase (MMP) mRNA expression were measured in the knee joint synovial fluid.

Results: The OC group had significantly shorter exercise times to exhaustion and withdrawal threshold according to the von Frey test, more severe knee joint swelling and subchondral bone erosion than the NC group, and reduced type II collagen and aggrecan expression in the articular cartilage. The OC group showed a significantly higher mRNA expression of inflammatory mediators, cytokines, and MMPs than the NC group. In OA rats, GGE03 administration reduced knee swelling and cartilage degradation and increased type II collagen and aggrecan expression in the articular cartilage. GGE03 administration reduced the expression of inducible nitric oxide synthase, cyclooxygenase-2, 5-lipoxygenase, interleukin (IL)-1β, IL-6, tumor necrosis factor-α, MMP-2, MMP-3, MMP-9, and MMP-13 in the synovia of the knee joints.

Conclusion: GGE03 administration is a useful supplementary agent for anti-OA treatment.

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蒸姜提取物改善碘乙酸钠诱导的骨关节炎大鼠的骨关节炎症状。

背景/目的：蒸姜提取物（GGE03）已被提议作为骨关节炎（OA）的补充治疗。另一方面，其疗效和机制尚不完全清楚。本研究考察了GGE03对碘乙酸钠（MIA）诱导的OA大鼠的抗骨关节炎作用。材料/方法：将大鼠分为正常对照组（NC）；OA对照组（OC）；OA+30、50或100 mg/kg体重/天GGE03组（O+G30、O+G50或O+G100）；OA+150 mg/kg BW/day甲基磺酰甲烷（MSM），阳性对照组（O+M）。SD大鼠分别口服GGE03或MSM 5周。第15天膝关节关节内注射MIA诱导OA。膝关节进行运动性能试验、疼痛行为试验、显微计算机断层扫描、组织学检查、免疫荧光染色。测定膝关节滑液中炎症介质、细胞因子和基质金属蛋白酶（MMP） mRNA表达。结果：根据von Frey试验，OC组的运动时间至疲劳和退出阈值明显短于NC组，膝关节肿胀和软骨下骨侵蚀较NC组严重，关节软骨中II型胶原和聚集蛋白表达减少。OC组炎症介质、细胞因子和MMPs mRNA表达明显高于NC组。在OA大鼠中，GGE03可减轻膝关节肿胀和软骨退化，并增加关节软骨中II型胶原和聚集蛋白的表达。GGE03可降低膝关节滑膜诱导型一氧化氮合酶、环氧合酶- 2,5 -脂氧合酶、白细胞介素(IL)-1β、IL-6、肿瘤坏死因子-α、MMP-2、MMP-3、MMP-9和MMP-13的表达。结论：GGE03是抗oa治疗的有效补充药物。

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来源期刊

Nutrition Research and Practice NUTRITION & DIETETICS-

CiteScore

3.50

自引率

4.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Nutrition Research and Practice (NRP) is an official journal, jointly published by the Korean Nutrition Society and the Korean Society of Community Nutrition since 2007. The journal had been published quarterly at the initial stage and has been published bimonthly since 2010. NRP aims to stimulate research and practice across diverse areas of human nutrition. The Journal publishes peer-reviewed original manuscripts on nutrition biochemistry and metabolism, community nutrition, nutrition and disease management, nutritional epidemiology, nutrition education, foodservice management in the following categories: Original Research Articles, Notes, Communications, and Reviews. Reviews will be received by the invitation of the editors only. Statements made and opinions expressed in the manuscripts published in this Journal represent the views of authors and do not necessarily reflect the opinion of the Societies.