Jianpi Qutan Decoction improves hepatic lipid metabolism in atherosclerosis mice via PPARα-CPT1α pathway regulation.

IF 2.5 3区生物学

Hereditas Pub Date : 2025-10-15 DOI:10.1186/s41065-025-00580-8

Xiaoyu Gao, Ying Hai, Dan Ma, Lisi Liu, Ying Pei, Jie Li, Wenping Wang

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引用次数: 0

Abstract

Objective: To investigate Jianpi Qutan Decoction (JPQT)'s effects on hepatic lipid metabolism via the PPARα-CPT1α pathway.

Methods: Eight male C57BL/6J mice served as controls; 32 ApoE^-/- mice were randomized into atherosclerosis (AS), atorvastatin calcium (AC), and low/medium/high-dose JPQT groups. Prior to the intervention, therapeutic targets of JPQT were analyzed using network pharmacology to provide a theoretical basis for subsequent experiments. The AS model was induced by a 12-week high-fat diet. Hepatic triglyceride (TG) and cholesterol (TC) were measured via GPO-PAP. Glucose tolerance (GTT) and insulin tolerance (ITT) were assessed. Pro-inflammatory cytokines were analyzed by ELISA/colorimetry. Fatty acid metabolism enzymes were evaluated using kits. PPARα-CPT1α pathway mRNA and protein expression were quantified via qPCR and Western blot.

Results: JPQT and AC reduced aortic plaque lipid deposition. JPQT significantly lowered hepatic TG/TC, blood glucose, insulin, and inflammation. It modulated fatty acid metabolism by promoting ACC phosphorylation, suppressing FAS and FFA while elevating FAβO, with dose-dependent efficacy. Additionally, JPQT upregulated PPARα, CPT1α, and ACOX1 mRNA and protein expression in the liver.

Conclusion: JPQT may improves lipid metabolism and reduces AS progression by activating the PPARα-CPT1α pathway, with higher doses yielding stronger effects.

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健脾祛痰汤通过调控PPARα-CPT1α通路改善动脉粥样硬化小鼠肝脏脂质代谢。

目的：探讨健脾祛痰汤通过PPARα-CPT1α通路对肝脏脂质代谢的影响。方法：8只雄性C57BL/6J小鼠作为对照；32只ApoE-/-小鼠随机分为动脉粥样硬化（AS）组、阿托伐他汀钙（AC）组和JPQT低/中/高剂量组。干预前利用网络药理学分析JPQT的治疗靶点，为后续实验提供理论基础。采用高脂饲料12周建立AS模型。GPO-PAP法测定肝脏甘油三酯（TG）和胆固醇（TC）。评估葡萄糖耐量（GTT）和胰岛素耐量（ITT）。采用ELISA/比色法分析促炎细胞因子。采用试剂盒检测脂肪酸代谢酶。通过qPCR和Western blot检测PPARα-CPT1α通路mRNA和蛋白的表达。结果：JPQT联合AC可减少主动脉斑块脂质沉积。JPQT显著降低肝脏TG/TC、血糖、胰岛素和炎症。它通过促进ACC磷酸化，抑制FAS和FFA，升高FAβO来调节脂肪酸代谢，具有剂量依赖性。此外，JPQT上调肝脏中PPARα、CPT1α和ACOX1 mRNA和蛋白的表达。结论：JPQT可能通过激活PPARα-CPT1α途径改善脂质代谢，减缓AS进展，且剂量越大作用越强。

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来源期刊

Hereditas Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.80

自引率

3.70%

发文量

期刊介绍： For almost a century, Hereditas has published original cutting-edge research and reviews. As the Official journal of the Mendelian Society of Lund, the journal welcomes research from across all areas of genetics and genomics. Topics of interest include human and medical genetics, animal and plant genetics, microbial genetics, agriculture and bioinformatics.