{"title":"Workflow for efficiently isolating microspore cultures of different rice genotypes by optimizing the callus induction medium.","authors":"Zhiwei Chen, Guimei Guo, Shuwei Zhang, Ting He, Shiji Feng, Chenghong Liu, Yu Wang, Longhua Zhou","doi":"10.3389/fpls.2025.1662463","DOIUrl":null,"url":null,"abstract":"<p><p>As one of the most important staple foods in the world, rice plays a key role in global food security. Doubled haploid technology based on isolated microspore culture can shorten the time taken for rice breeding programs. However, this technology still faces many problems, such as genotypic dependency, low culture efficiency, and a shortage of skilled workers. In this study, 15 rice genotypes, comprising 12 <i>japonica</i> genotypes and 3 <i>indica</i> genotypes, were randomly selected for microspore culture research, and the effects of different callus induction media (CIMs) on callus induction were compared and the related plant regenerations were also shown. The results showed that maltose was the optimal carbon source and the CIM III was the best for callus induction by comparing the number of rice genotypes that could be induced to form calli and the callus yields. For plant differentiation, 12 of the 14 rice genotypes regenerated green seedlings, all of which were <i>japonica</i> rice genotypes. Ploidy identification showed that the spontaneous doubling rate of regenerated seedlings from isolated microspore cultures ranged from 14.3 to 98%, which was higher than that observed in anther cultures. In conclusion, this study established an isolated microspore culture method that is suitable for different rice genotypes, providing more options for using doubled haploid technology in rice.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1662463"},"PeriodicalIF":4.1000,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12518337/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Plant Science","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3389/fpls.2025.1662463","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
As one of the most important staple foods in the world, rice plays a key role in global food security. Doubled haploid technology based on isolated microspore culture can shorten the time taken for rice breeding programs. However, this technology still faces many problems, such as genotypic dependency, low culture efficiency, and a shortage of skilled workers. In this study, 15 rice genotypes, comprising 12 japonica genotypes and 3 indica genotypes, were randomly selected for microspore culture research, and the effects of different callus induction media (CIMs) on callus induction were compared and the related plant regenerations were also shown. The results showed that maltose was the optimal carbon source and the CIM III was the best for callus induction by comparing the number of rice genotypes that could be induced to form calli and the callus yields. For plant differentiation, 12 of the 14 rice genotypes regenerated green seedlings, all of which were japonica rice genotypes. Ploidy identification showed that the spontaneous doubling rate of regenerated seedlings from isolated microspore cultures ranged from 14.3 to 98%, which was higher than that observed in anther cultures. In conclusion, this study established an isolated microspore culture method that is suitable for different rice genotypes, providing more options for using doubled haploid technology in rice.
期刊介绍：
In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches.
Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.
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