Developing a dichlorosalicylaldehyde-derived fluorescent probe for monitoring glutathione in a cellular pulmonary ventilation model.

IF 4.2 3区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Frontiers in Chemistry Pub Date : 2025-09-30 eCollection Date: 2025-01-01 DOI:10.3389/fchem.2025.1698116

Xiao Zhou, Lei Zhang, Yuwen Lao, Bin Zhou, Zhongquan Zhu

引用次数: 0

Abstract

Herein, a fluorescent probe, DSNBD-GSH, was developed from dichlorosalicylaldehyde to monitor glutathione (GSH) in a cellular pulmonary ventilation model. Both the normoxia and hypoxia conditions were evaluated. DSNBD-GSH responded to GSH in a dosage-dependent manner with a fluorescence signal at 478 nm under the excitation of 365 nm. The solution tests indicated that DSNBD-GSH had relatively high sensitivity for GSH, and the photophysical properties were stable in various conditions. Other advantages included rapid response, high selectivity, and low cytotoxicity. Most significantly, monitoring the GSH level in both hypoxia and recovery status allows for visualization of ventilation-related redox changes. This work highlights a referenceable case for improving the pulmonary ventilation approach during the perioperative period.

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二氯水杨醛衍生的荧光探针用于监测细胞肺通气模型中的谷胱甘肽。

本文以二氯水杨醛为原料制备了一种荧光探针DSNBD-GSH，用于监测细胞肺通气模型中的谷胱甘肽（GSH）。评估正常氧合和缺氧条件。DSNBD-GSH对GSH的响应呈剂量依赖性，在365 nm的激发下，在478 nm处发出荧光信号。溶液试验表明，DSNBD-GSH对谷胱甘肽具有较高的敏感性，在各种条件下光物理性质稳定。其他优点包括快速反应，高选择性和低细胞毒性。最重要的是，在缺氧和恢复状态下监测GSH水平可以可视化通风相关的氧化还原变化。本研究为围手术期改善肺通气入路提供了一个可借鉴的案例。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Chemistry Chemistry-General Chemistry

CiteScore

8.50

自引率

3.60%

发文量

1540

审稿时长

12 weeks

期刊介绍： Frontiers in Chemistry is a high visiblity and quality journal, publishing rigorously peer-reviewed research across the chemical sciences. Field Chief Editor Steve Suib at the University of Connecticut is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to academics, industry leaders and the public worldwide. Chemistry is a branch of science that is linked to all other main fields of research. The omnipresence of Chemistry is apparent in our everyday lives from the electronic devices that we all use to communicate, to foods we eat, to our health and well-being, to the different forms of energy that we use. While there are many subtopics and specialties of Chemistry, the fundamental link in all these areas is how atoms, ions, and molecules come together and come apart in what some have come to call the “dance of life”. All specialty sections of Frontiers in Chemistry are open-access with the goal of publishing outstanding research publications, review articles, commentaries, and ideas about various aspects of Chemistry. The past forms of publication often have specific subdisciplines, most commonly of analytical, inorganic, organic and physical chemistries, but these days those lines and boxes are quite blurry and the silos of those disciplines appear to be eroding. Chemistry is important to both fundamental and applied areas of research and manufacturing, and indeed the outlines of academic versus industrial research are also often artificial. Collaborative research across all specialty areas of Chemistry is highly encouraged and supported as we move forward. These are exciting times and the field of Chemistry is an important and significant contributor to our collective knowledge.