Zinc(II) and Copper(II) Binding of a MUC7 Peptide Fragment: Coordination, Thermodynamic Stability, and Antimicrobial Potency

IF 2 4区 化学 Q3 CHEMISTRY, INORGANIC & NUCLEAR
Alicja Krynicka, Wojciech Lizak, Klaudia Szarszoń, Anna Ślusarczyk, Tomasz Janek, Joanna Wątły
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Abstract

Metal ions play a key role in regulating antimicrobial peptides (AMPs), either inducing or enhancing their effects against pathogens. In human saliva, AMPs, including fragments of the glycoprotein MUC7, form a natural defense against oral and systemic infections. This study investigates a 20-residue MUC7-derived peptide containing three histidine residues as potential coordination sites for divalent metal ions. Complexes are analyzed using electrospray ionization mass spectrometry, potentiometry, and spectroscopic methods: ultraviolet–visible spectroscopy, circular dichroism, and electron paramagnetic resonance, together with antimicrobial assays. Cu(II) coordination did not significantly alter secondary structure or improve antimicrobial properties. In contrast, Zn(II) induced conformational changes under alkaline conditions, correlating with slightly enhanced antimicrobial performance. Proline, tyrosine, and phenylalanine residues likely modulate metal coordination through steric hindrance and conformational effects. These findings highlight the role of metal coordination in modulating AMP function. Although this particular MUC7-derived fragment exhibits only modest antimicrobial activity compared to other known fragments of this protein, its distinct metal-binding behavior suggests that it may serve an alternative biological role in the oral environment beyond direct pathogen inhibition.

Abstract Image

MUC7肽片段的锌(II)和铜(II)结合:配位、热力学稳定性和抗菌效力
金属离子在调节抗菌肽(AMPs)中发挥关键作用,诱导或增强其对病原体的作用。在人类唾液中,AMPs,包括糖蛋白MUC7的片段,形成了对口腔和全身感染的天然防御。本研究研究了一种含有3个组氨酸残基作为二价金属离子潜在配位位的muc7衍生肽。配合物的分析使用电喷雾电离质谱,电位测定法和光谱方法:紫外-可见光谱,圆二色,电子顺磁共振,以及抗菌分析。铜(II)配位没有显著改变二级结构或改善抗菌性能。相比之下,Zn(II)在碱性条件下引起构象变化,与抗菌性能略有提高相关。脯氨酸、酪氨酸和苯丙氨酸残基可能通过位阻和构象效应调节金属配位。这些发现强调了金属配位在调节AMP功能中的作用。尽管与其他已知的muc7蛋白片段相比,这种特殊的muc7衍生片段仅表现出适度的抗菌活性,但其独特的金属结合行为表明,它可能在口腔环境中发挥替代的生物学作用,而不是直接抑制病原体。
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来源期刊
European Journal of Inorganic Chemistry
European Journal of Inorganic Chemistry 化学-无机化学与核化学
CiteScore
4.30
自引率
4.30%
发文量
419
审稿时长
1.3 months
期刊介绍: The European Journal of Inorganic Chemistry (2019 ISI Impact Factor: 2.529) publishes Full Papers, Communications, and Minireviews from the entire spectrum of inorganic, organometallic, bioinorganic, and solid-state chemistry. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies. The following journals have been merged to form the two leading journals, European Journal of Inorganic Chemistry and European Journal of Organic Chemistry: Chemische Berichte Bulletin des Sociétés Chimiques Belges Bulletin de la Société Chimique de France Gazzetta Chimica Italiana Recueil des Travaux Chimiques des Pays-Bas Anales de Química Chimika Chronika Revista Portuguesa de Química ACH—Models in Chemistry Polish Journal of Chemistry The European Journal of Inorganic Chemistry continues to keep you up-to-date with important inorganic chemistry research results.
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