Hend S. Magar, Aditya Shekhar, Ursula Bilitewski, Rabeay Y. A. Hassan
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引用次数: 0
Abstract
α-Hemolysin, produced by Staphylococcus aureus, is one of the most potent secreted toxins. It directly destroys host cell membranes by forming transmembrane pores, ultimately leading to severe health complications. Accordingly, there is an urgent need to rapidly detect contaminated samples with such toxins. Here, disposable immunosensing system was designed using a selective anti-α-hemolysin antibody assembled onto nanostructured disposable sensor chips modified with AuNPs-Co3O4-CuO@MWCNTs nanocomposite. Bimetal oxides of cobalt and copper were chemically synthesized and integrated with the multiwalled carbon nanotubes and gold nanoparticles to yield the desired sensing platform that provides high electrocatalytic and high sensing performance. Consequently, the electrochemical assay was systematically optimized, with several analytical parameters evaluated, including the nanocomposite composition ratios, antibody loading concentration, incubation time between the exotoxin and its specific antibody, and the detectable concentration range of the target toxin. Furthermore, the nanostructured materials were comprehensively characterized through a combination of physical and chemical techniques, such as X-ray diffraction (XRD), Raman spectroscopy, electron microscopy, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). Eventually, a calibration curve with a wide dynamic range was obtained, demonstrating high sensitivity with a limit of detection of 0.01 ng/mL. The applicability of the newly designed immunosensors for real quantitative analysis in food samples was investigated, showing high recovery rates (from 96.0 to 107%) towards the target analyte.
期刊介绍：
Advanced Composites and Hybrid Materials is a leading international journal that promotes interdisciplinary collaboration among materials scientists, engineers, chemists, biologists, and physicists working on composites, including nanocomposites. Our aim is to facilitate rapid scientific communication in this field.
The journal publishes high-quality research on various aspects of composite materials, including materials design, surface and interface science/engineering, manufacturing, structure control, property design, device fabrication, and other applications. We also welcome simulation and modeling studies that are relevant to composites. Additionally, papers focusing on the relationship between fillers and the matrix are of particular interest.
Our scope includes polymer, metal, and ceramic matrices, with a special emphasis on reviews and meta-analyses related to materials selection. We cover a wide range of topics, including transport properties, strategies for controlling interfaces and composition distribution, bottom-up assembly of nanocomposites, highly porous and high-density composites, electronic structure design, materials synergisms, and thermoelectric materials.
Advanced Composites and Hybrid Materials follows a rigorous single-blind peer-review process to ensure the quality and integrity of the published work.
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