Comprehensive transcriptomic profiling of dynamic endometrial alterations during different implantation stages in goats (Capra hircus)

IF 2.9 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of animal science Pub Date : 2025-10-15 DOI:10.1093/jas/skaf347

Junyin Zhao, Shengnan Zhao, Yangshen Zhang, Jikang Zheng, Yuhao Liao, Mingming Wang, Xingqiang Fang, Zhipeng Sun, Yongju Zhao

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引用次数: 0

Abstract

The establishment of endometrial receptivity constitutes a multifaceted biological process orchestrated by intricate regulatory networks, representing a critical determinant for successful embryo implantation and pregnancy outcomes. This study systematically characterized the dynamic landscapes of non-coding RNA (ncRNA) and mRNA in the goat endometrium across distinct implantation stages. Endometrial tissues from goats at gestational days 10 (D10, n = 4), 17 (D17, n = 4), and pregnancy failure at gestational day 17 (PF, n = 3) were collected; in addition, three samples from each group (n = 3) were selected for transcriptomic sequencing of mRNA, lncRNA, circRNA, and miRNA. The receptive endometrium (D17) exhibited enhanced glandular development, mature pinopodes, elevated estrogen and progesterone levels, and a reduced estrogen/progesterone ratio, along with upregulated markers (VEGF, OPN, IGF1R). Transcriptome profiling identified 6,104 differentially expressed mRNAs (DEmRNAs), 485 lncRNAs (DElncRNAs), 1,694 circRNAs (DEcircRNAs), and 203 miRNAs (DEmiRNAs) across stages. Functional enrichment analysis highlighted the pivotal roles of MAPK, TNF, and RAS signaling pathways in establishing endometrial receptivity, with hub genes including ISG15, CXCL10, IFI6, and RSAD2 contributing significantly to the development of uterine receptivity in goats. Furthermore, ncRNAs such as MSTRG774.1, novel_circ_000145, novel_circ_000257, and chi-miR-348-3p exhibited specific or elevated expression patterns during the receptive phase, suggesting their potential regulatory functions in maintaining the endometrial receptivity microenvironment. Moreover, an integrated competing endogenous RNA (ceRNA) network was constructed to elucidate potential interactions between ncRNAs and mRNAs, offering novel insights into the molecular regulatory mechanisms underlying endometrial receptivity in ruminants.

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山羊不同着床阶段动态子宫内膜改变的综合转录组学分析

子宫内膜容受性的建立是一个由复杂的调控网络精心策划的多方面生物学过程，是胚胎成功着床和妊娠结局的关键决定因素。本研究系统地描述了不同着床阶段山羊子宫内膜中非编码RNA （ncRNA）和mRNA的动态变化。收集山羊妊娠第10天（D10, n = 4）、第17天（D17, n = 4）和妊娠第17天（PF, n = 3）的子宫内膜组织；此外，每组各取3个样本（n = 3），对mRNA、lncRNA、circRNA和miRNA进行转录组测序。受体子宫内膜（D17）表现出腺体发育增强，足部成熟，雌激素和孕激素水平升高，雌激素/孕激素比例降低，以及标志物（VEGF， OPN, IGF1R）上调。转录组分析鉴定了6104个差异表达mrna (demrna)， 485个lncRNAs (DElncRNAs)， 1694个circRNAs （DEcircRNAs）和203个miRNAs （DEmiRNAs）。功能富集分析强调了MAPK、TNF和RAS信号通路在建立子宫内膜容受性中的关键作用，其中ISG15、CXCL10、IFI6和RSAD2等枢纽基因在山羊子宫容受性的发展中起着重要作用。此外，ncRNAs如MSTRG774.1、novel_circ_000145、novel_circ_000257和chi-miR-348-3p在接受期表现出特异性或升高的表达模式，表明它们在维持子宫内膜容受性微环境方面具有潜在的调节功能。此外，我们构建了一个整合的竞争内源性RNA （ceRNA）网络来阐明ncRNAs和mrna之间潜在的相互作用，为反刍动物子宫内膜容受性的分子调控机制提供了新的见解。

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来源期刊

Journal of animal science 农林科学-奶制品与动物科学

CiteScore

4.80

自引率

12.10%

发文量

1589

审稿时长

3 months

期刊介绍： The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.