Electrochemical quantification of intracellular hydrogen peroxide using cobalt phthalocyanine modified carbon nanopipettes

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-10-13 DOI:10.1016/j.snb.2025.138983

Dehui Yu , Hongli Cao , Yingjie Zhao , Rujia Liu , Xiaoyue Shen , Dengchao Wang

{"title":"Electrochemical quantification of intracellular hydrogen peroxide using cobalt phthalocyanine modified carbon nanopipettes","authors":"Dehui Yu , Hongli Cao , Yingjie Zhao , Rujia Liu , Xiaoyue Shen , Dengchao Wang","doi":"10.1016/j.snb.2025.138983","DOIUrl":null,"url":null,"abstract":"<div><div>As the most stable member of the reactive oxygen species (ROS), hydrogen peroxide (H₂O₂) serves as both the key signaling molecule and pathological mediator. Real-time monitoring and quantitative analysis of H₂O₂ at the individual cell level are highly needed to fully resolve the cellular heterogeneity and uncover H₂O₂ roles in cellular metabolism and disease progression. Herein, we reported an electrochemical nanosensor for H₂O₂ detection based on sulfonated cobalt phthalocyanine modified carbon nanopipettes, and achieved real-time monitoring and quantification of H₂O₂ in a single living HeLa cell. Meanwhile, the numerical simulation was also conducted to help understand the different current trends when monitoring the intracellular H<sub>2</sub>O<sub>2</sub> in different cells. This work provided a new platform for single-cell electrochemical detection of H₂O₂, promoting the understanding of ROS related cellular processes under oxidative stress.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138983"},"PeriodicalIF":3.7000,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors and Actuators B: Chemical","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0925400525017599","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

As the most stable member of the reactive oxygen species (ROS), hydrogen peroxide (H₂O₂) serves as both the key signaling molecule and pathological mediator. Real-time monitoring and quantitative analysis of H₂O₂ at the individual cell level are highly needed to fully resolve the cellular heterogeneity and uncover H₂O₂ roles in cellular metabolism and disease progression. Herein, we reported an electrochemical nanosensor for H₂O₂ detection based on sulfonated cobalt phthalocyanine modified carbon nanopipettes, and achieved real-time monitoring and quantification of H₂O₂ in a single living HeLa cell. Meanwhile, the numerical simulation was also conducted to help understand the different current trends when monitoring the intracellular H₂O₂ in different cells. This work provided a new platform for single-cell electrochemical detection of H₂O₂, promoting the understanding of ROS related cellular processes under oxidative stress.

Abstract Image

查看原文本刊更多论文

利用钴酞菁修饰碳纳米管对细胞内过氧化氢进行电化学定量

过氧化氢（h2o2）作为活性氧（ROS）中最稳定的成员，既是关键的信号分子，也是病理介质。在单个细胞水平上实时监测和定量分析H₂O₂是非常必要的，以充分解决细胞异质性，揭示H₂O₂在细胞代谢和疾病进展中的作用。在此，我们报道了一种基于磺化钴酞菁修饰的纳米碳吸管的电化学纳米传感器，用于检测H₂O₂，并实现了单个活HeLa细胞中H₂O₂的实时监测和定量。同时，通过数值模拟，了解不同细胞内H2O2监测的不同趋势。本研究为单细胞h2o2的电化学检测提供了新的平台，促进了对氧化应激下ROS相关细胞过程的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.