Phenotypic Function of Legionella pneumophila Type I-F CRISPR-Cas.

IF 4.1

Biomedical and environmental sciences : BES Pub Date : 2025-09-20 DOI:10.3967/bes2025.107

Ting Mo, Hong Yu Ren, Xian Xian Zhang, Yun Wei Lu, Zhong Qiu Teng, Xue Zhang, Lu Peng Dai, Ling Hou, Na Zhao, Jia He, Tian Qin

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Abstract

Objective: CRISPR-Cas protects bacteria from exogenous DNA invasion and is associated with bacterial biofilm formation and pathogenicity.

Methods: We analyzed the type I-F CRISPR-Cas system of Legionella pneumophila WX48, including Cas1, Cas2-Cas3, Csy1, Csy2, Csy3, and Cas6f, along with downstream CRISPR arrays. We explored the effects of the CRISPR-Cas system on the in vitro growth, biofilm-forming ability, and pathogenicity of L. pneumophila through constructing gene deletion mutants.

Results: The type I-F CRISPR-Cas system did not affect the in vitro growth of wild-type or mutant strains. The biofilm formation and intracellular proliferation of the mutant strains were weaker than those of the wild type owing to the regulation of type IV pili and Dot/Icm type IV secretion systems. In particular, Cas6f deletion strongly inhibited these processes.

Conclusion: The type I-F CRISPR-Cas system may reduce biofilm formation and intracellular proliferation in L. pneumophila.

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嗜肺军团菌I-F型CRISPR-Cas的表型功能。

目的：CRISPR-Cas保护细菌免受外源DNA入侵，并与细菌生物膜形成和致病性有关。方法：分析嗜肺军团菌WX48的I-F型CRISPR- cas系统，包括Cas1、Cas2-Cas3、Csy1、Csy2、Csy3和Cas6f，以及下游的CRISPR阵列。我们通过构建基因缺失突变体，探索CRISPR-Cas系统对嗜肺乳杆菌体外生长、生物膜形成能力和致病性的影响。结果：I-F型CRISPR-Cas系统对野生型和突变株的体外生长没有影响。由于IV型菌毛和Dot/Icm型分泌系统的调控，突变菌株的生物膜形成和细胞内增殖较野生型弱。特别是，Cas6f缺失强烈抑制了这些过程。结论：I-F型CRISPR-Cas系统可减少嗜肺乳杆菌的生物膜形成和细胞内增殖。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biomedical and environmental sciences : BES

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