Genetic diversity of Echinococcus multilocularis from red foxes and humans in northern and northeastern Poland investigated using the microsatellite EmsB.

IF 2.6 4区医学 Q3 INFECTIOUS DISEASES

Infection Genetics and Evolution Pub Date : 2025-10-12 DOI:10.1016/j.meegid.2025.105843

Paweł Gładysz, Małgorzata Samorek-Pieróg, Jacek Karamon, Krzysztof Rębała, Małgorzata Sulima, Dariusz Zadrożny, Anna Lass

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引用次数: 0

Abstract

Background: Echinococcus multilocularis is the causative agent of alveolar echinococcosis (AE). We explored the diversity of EmsB profiles of E. multilocularis from red foxes and humans in northern and northeastern Poland, with a particular focus on autochthonous Asian variants.

Methods: We investigated 263 adult tapeworms from 59 red foxes hunted in selected districts of three voivodships and ten metacestodes extracted from AE patients who never visited Asia. We conducted hierarchical clustering of the obtained EmsB profiles combined with a custom Asian reference dataset and interpreted the resulting phenogram by applying the standard genetic distance threshold (GDT) of 0.08 and Dynamic Tree Cut (DTC).

Results: The GDT divided the 273 profiles into six units, with Pol-B being the most frequent (220/273, 81 %) and widespread variant. DTC grouped the profiles into three phenons (PH). Eight out of ten people got infected with the predominant variant, PH-2/Pol-B. Among the 273 samples, thirty-six (13 %) matched the Asian reference set, including metacestodes from a Lithuanian patient and a Polish patient.

Conclusions: Genetically extra-European variants reach as far north as Pomorskie Voivodship. They likely come from East Asia. The autochthonous AE case with an Asian profile confirms that such tapeworms have penetrated the synanthropic cycle.

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用微卫星EmsB对波兰北部和东北部地区红狐和人多房棘球蚴的遗传多样性进行了研究。

背景：多房棘球蚴是肺泡棘球蚴病（AE）的病原体。我们探索了波兰北部和东北部红狐和人类多房棘猴EmsB谱的多样性，特别关注亚洲本土变异。方法：从3个省选定地区捕获的59只红狐中提取263条成虫，并从从未去过亚洲的AE患者中提取10种囊绦虫。我们结合自定义的亚洲参考数据集对获得的EmsB谱进行了分层聚类，并通过0.08的标准遗传距离阈值（GDT）和动态树切（DTC）来解释得到的表型图。结果：GDT将273个基因型分为6个单位，其中Pol-B是最常见的（220/273,81 %）和广泛变异。DTC将这些剖面分为三个表型（pH）。10人中有8人感染了主要变种PH-2/Pol-B。在273个样本中，36个（13 %）与亚洲参考集匹配，包括来自立陶宛患者和波兰患者的元跖骨。结论：基因上的非欧洲变异最远可到达波莫尔斯基省北部。他们可能来自东亚。具有亚洲特征的本土AE病例证实，这种绦虫已经穿透了共胞循环。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Infection Genetics and Evolution 医学-传染病学

CiteScore

8.40

自引率

0.00%

发文量

215

审稿时长

82 days

期刊介绍： (aka Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases -- MEEGID) Infectious diseases constitute one of the main challenges to medical science in the coming century. The impressive development of molecular megatechnologies and of bioinformatics have greatly increased our knowledge of the evolution, transmission and pathogenicity of infectious diseases. Research has shown that host susceptibility to many infectious diseases has a genetic basis. Furthermore, much is now known on the molecular epidemiology, evolution and virulence of pathogenic agents, as well as their resistance to drugs, vaccines, and antibiotics. Equally, research on the genetics of disease vectors has greatly improved our understanding of their systematics, has increased our capacity to identify target populations for control or intervention, and has provided detailed information on the mechanisms of insecticide resistance. However, the genetics and evolutionary biology of hosts, pathogens and vectors have tended to develop as three separate fields of research. This artificial compartmentalisation is of concern due to our growing appreciation of the strong co-evolutionary interactions among hosts, pathogens and vectors. Infection, Genetics and Evolution and its companion congress [MEEGID](http://www.meegidconference.com/) (for Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases) are the main forum acting for the cross-fertilization between evolutionary science and biomedical research on infectious diseases. Infection, Genetics and Evolution is the only journal that welcomes articles dealing with the genetics and evolutionary biology of hosts, pathogens and vectors, and coevolution processes among them in relation to infection and disease manifestation. All infectious models enter the scope of the journal, including pathogens of humans, animals and plants, either parasites, fungi, bacteria, viruses or prions. The journal welcomes articles dealing with genetics, population genetics, genomics, postgenomics, gene expression, evolutionary biology, population dynamics, mathematical modeling and bioinformatics. We also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services .