Acute Sleep Deprivation Suppresses Sleep Spindles in Mice.

IF 3.4 2区医学 Q2 CLINICAL NEUROLOGY

Nature and Science of Sleep Pub Date : 2025-10-09 eCollection Date: 2025-01-01 DOI:10.2147/NSS.S549263

Yan Xu, Miaoqin Tan, Dongcai Li, Chenxi Zhang

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Abstract

Introduction: Sleep spindles and cortical coherence are key electrophysiological signatures of thalamocortical communication and large-scale neural synchrony during non-REM sleep. This study aimed to evaluate the effects of acute 12-hour sleep deprivation on spindle dynamics and cortical coherence across frequency bands in mice.

Methods: Sixteen adult male C57BL/6J (8 per group) mice were randomly assigned to either a natural sleep (NS) or sleep deprivation (SD) group. We recorded bilateral frontal EEGs continuously for 12 hours following intervention. Sleep spindles (8-16 Hz) were automatically detected, and interhemispheric coherence from delta to gamma bands was computed using Welch's method.

Results: Compared to NS mice, SD mice showed a significant reduction in frontal spindle count (61 ± 14 vs 103 ± 12 spindles; mean ± SEM; p = 0.027) and amplitude (47 ± 6 µV vs 68 ± 5 µV; p = 0.002). Delta (0.5-4 Hz) and theta (4-8 Hz) interhemispheric coherence showed non-significant decreasing trends in SD mice (p > 0.05), whereas alpha (8-13 Hz), beta (13-30 Hz), and gamma (30-40 Hz) coherence did not differ.

Conclusion: Acute sleep deprivation significantly impairs spindle generation. It also resulted in a trend toward reduced low-frequency cortical coherence, though this change was not statistically significant.

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急性睡眠剥夺抑制小鼠睡眠纺锤波。

睡眠纺锤波和皮层相干性是非快速眼动睡眠期间丘脑皮质通讯和大规模神经同步的关键电生理特征。本研究旨在评估急性12小时睡眠剥夺对小鼠纺锤体动力学和皮层各频段相干性的影响。方法：将16只成年雄性C57BL/6J小鼠（每组8只）随机分为自然睡眠（NS）组和睡眠剥夺（SD）组。干预后连续记录双侧额叶脑电图12小时。自动检测睡眠纺锤波（8-16 Hz），并使用Welch方法计算从delta到gamma波段的半球间相干性。结果：与NS小鼠相比，SD小鼠额叶纺锤体数量（61±14 vs 103±12;mean±SEM; p = 0.027）和振幅（47±6µV vs 68±5µV; p = 0.002）显著减少。SD小鼠的δ (0.5-4 Hz)和θ (4-8 Hz)半球间相干性呈无显著性下降趋势（p < 0.05），而α (8-13 Hz)、β (13-30 Hz)和γ (30-40 Hz)半球间相干性无显著性差异。结论：急性睡眠剥夺显著损害纺锤体生成。它还导致了低频皮层一致性降低的趋势，尽管这种变化在统计上并不显著。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature and Science of Sleep Neuroscience-Behavioral Neuroscience

CiteScore

5.70

自引率

5.90%

发文量

245

审稿时长

16 weeks

期刊介绍： Nature and Science of Sleep is an international, peer-reviewed, open access journal covering all aspects of sleep science and sleep medicine, including the neurophysiology and functions of sleep, the genetics of sleep, sleep and society, biological rhythms, dreaming, sleep disorders and therapy, and strategies to optimize healthy sleep. Specific topics covered in the journal include: The functions of sleep in humans and other animals Physiological and neurophysiological changes with sleep The genetics of sleep and sleep differences The neurotransmitters, receptors and pathways involved in controlling both sleep and wakefulness Behavioral and pharmacological interventions aimed at improving sleep, and improving wakefulness Sleep changes with development and with age Sleep and reproduction (e.g., changes across the menstrual cycle, with pregnancy and menopause) The science and nature of dreams Sleep disorders Impact of sleep and sleep disorders on health, daytime function and quality of life Sleep problems secondary to clinical disorders Interaction of society with sleep (e.g., consequences of shift work, occupational health, public health) The microbiome and sleep Chronotherapy Impact of circadian rhythms on sleep, physiology, cognition and health Mechanisms controlling circadian rhythms, centrally and peripherally Impact of circadian rhythm disruptions (including night shift work, jet lag and social jet lag) on sleep, physiology, cognition and health Behavioral and pharmacological interventions aimed at reducing adverse effects of circadian-related sleep disruption Assessment of technologies and biomarkers for measuring sleep and/or circadian rhythms Epigenetic markers of sleep or circadian disruption.