Efecto protector de propóleo frente al daño oxidativo inducido con peróxido de hidrógeno en células mononucleares de sangre periférica cultivadas in vitro mediante conteo celular

IF 0.5 Q3 Health Professions

Revista Colombiana de Reumatologia Pub Date : 2025-10-01 Epub Date: 2024-08-01 DOI:10.1016/j.rcreu.2024.05.003

Luz Elena Triana Vidal, Armando Lucumi Moreno, Ivonne Valeria Ortiz

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引用次数: 0

Abstract

Introduction

Oxidative stress occurs when there is an imbalance between the production of reactive oxygen species (ROS) and cellular antioxidant activity. Overproduction of ROS causes oxidative damage to major macromolecules, alters homeostasis and leads to the generation of different pathologies. At present, there is no totally effective treatment to counteract these diseases involved with oxidative stress, so it is necessary to investigate new treatment alternatives that suppress the ROS generated. One promising alternative is the use of plant extracts, which have demonstrated a potent antioxidant effect. Efficient cells for the study of pathological diseases related to reactive oxygen species are T lymphocytes, which share systems with neurons such as: the dopaminergic system, death signaling and survival. In vitro lymphocytes are an optimal model for the evaluation of oxidative mechanisms.

Objective

To determine the protective effect of ethanolic extract of propolis against oxidative damage induced by hydrogen peroxide (H₂O₂) in human lymphocytes in vitro, by means of the cell viability test with trypan blue.

Results

Anova test shows that the concentrations of 0.0225 and 0.045 mg/mL of ethanolic extract of propolis, present protective activity against cell damage caused by H₂O₂.

Conclusion

This study proposes the ethanolic extract of propolis as a potential pharmacological, useful for treatments against autoimmune disorders.

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在体外培养的外周血单核细胞中，通过细胞计数，丙烯对过氧化氢引起的氧化损伤起到保护作用

当活性氧（ROS）的产生和细胞抗氧化活性之间存在不平衡时，就会发生氧化应激。ROS的过量产生导致主要大分子的氧化损伤，改变体内平衡，导致不同病理的产生。目前还没有完全有效的治疗方法来对抗这些与氧化应激有关的疾病，因此有必要研究新的治疗方案来抑制ROS的产生。一种有希望的替代方法是使用植物提取物，它已被证明具有强大的抗氧化作用。研究与活性氧有关的病理疾病的有效细胞是T淋巴细胞，它与神经元共享系统，如：多巴胺能系统、死亡信号和生存。体外淋巴细胞是评价氧化机制的最佳模型。目的通过台盼蓝细胞活力试验，探讨蜂胶醇提物对过氧化氢（H2O2）诱导的体外人淋巴细胞氧化损伤的保护作用。结果sanova实验表明，0.0225和0.045 mg/mL浓度的蜂胶乙醇提取物对H2O2对细胞的损伤具有保护作用。结论蜂胶乙醇提取物具有潜在的治疗自身免疫性疾病的药理作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Revista Colombiana de Reumatologia Medicine-Rheumatology

CiteScore

0.80

自引率

0.00%

发文量

期刊介绍： The Colombian Journal of Rheumatology (Revista Colombiana de Reumatología) is the official organ of the Colombian Association of Rheumatology (Asociación Colombiana de Reumatología) and the Central American, Caribbean and Andean Association of Rheumatology (Asociación Centroamericana Caribe Andina de Reumatología) - ACCA. It was created in December 1993 with the purpose of disseminating scientific information derived from primary and secondary research and presenting cases coming from the practice of Rheumatology in Latin America. Since its foundation, the Journal has been characterized by its plurality with subjects of all rheumatic and osteomuscular pathologies, in the form of original articles, historical articles, economic evaluations, and articles of reflection and education in Medicine. It covers an extensive area of topics ranging from the broad spectrum of the clinical aspects of rheumatology and related areas in autoimmunity (both in pediatric and adult pathologies), to aspects of basic sciences. It is an academic tool for the different members of the academic and scientific community at their different levels of training, from undergraduate to post-doctoral degrees, managing to integrate all actors inter and trans disciplinarily. It is intended for rheumatologists, general internists, specialists in related areas, and general practitioners in the country and abroad. It has become an important space in the work of all rheumatologists from Central and South America.