WarmStart colorimetric reverse transcription-loop-mediated isothermal amplification assay for the visual, sensitive, and specific detection of epizootic haemorrhagic disease virus in China

IF 1.9 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2025-10-12 DOI:10.1016/j.mimet.2025.107292

Zhanhong Li , Heng Yang , Zhuoran Li , Zhenxing Yang , Huachun Li , Defang Liao

引用次数: 0

Abstract

A pan-specific colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to detect Chinese epizootic haemorrhagic disease virus strains (EHDVs) inaugurally. The assay could be completed in 45 min at 64 °C, and could detect as few as 15 copies of EHDV RNA, the coincidence rates of the established RT-LAMP assay and previously reported qRT-PCR were more than 86.7 % in the clinical evaluation.

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WarmStart比色法逆转录环介导的等温扩增法用于中国家畜出血病病毒的视觉、敏感和特异性检测

首次建立了反转录环介导等温扩增（RT-LAMP）泛特异性比色法检测中国流行性出血病病毒（edhv）的方法。该方法在64℃下45 min即可完成，最多可检测到15个EHDV RNA拷贝，在临床评价中，所建立的RT-LAMP方法与先前报道的qRT-PCR的符合率超过86.7%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.