An assessment of the accuracy of morphological techniques for identifying Lucilia cuprina and Lucilia sericata (Diptera: Calliphoridae).

Abstract

Aims: To assess the accuracy of the morphological identification of Lucilia cuprina and Lucilia sericata by using molecular analysis as a reference standard test, and to describe the seasonality of these species.

Methods: A convenience sample of L. cuprina and L. sericata flies was caught on eight farms from across New Zealand and stored at room temperature in 70% alcohol. They were first morphologically identified using published keys and then molecularly identified using primers to amplify the 28S rRNA region of the nuclear genome. The accuracy of the morphological identification was then estimated for each species using the molecular identification as a reference standard test. The correctness of the published keys was also tested by re-examining a sample of misidentified flies using enhanced magnification and photography.

Results: The accuracy of the morphological identification for L. cuprina was 0.66 (95% CI = 0.58-0.73) and for L. sericata was 0.7 (95% CI = 0.62-0.77). There was no evidence for a difference in accuracy between species (p = 0.56), and re-examination of the misidentified flies found no faults in the published keys. The study confirmed that L. cuprina has a longer season of activity than L. sericata.

Conclusions: These results emphasise the need to use molecular methods to confirm the identification of these species, especially when dealing with large, stored collections, rather than to rely on morphological identification alone.

Clinical relevance: Without accurate fly identification and knowledge of insecticide resistance status, effective control and prevention of flystrike in New Zealand could be handicapped.