MLE/DHX9 Helicase Activity Is Required to Regulate the Expression of a Number of Tissue-Specific Genes on Chromosome 4 in Drosophila melanogaster.

IF 0.7 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Doklady Biochemistry and Biophysics Pub Date : 2025-10-13 DOI:10.1134/S1607672925601222

I A Zolin, A A Grigel, S G Georgieva, A N Krasnov, J V Nikolenko

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引用次数: 0

Abstract

DHX9 helicase and its ortholog MLE in D. melanogaster participate in different stages of gene expression. Both helicases are important for the formation and functioning of the nervous system in humans and D. melanogaster, respectively. However, the role of helicase activity of DHX9 and MLE in the regulation of gene expression has been poorly studied, and the existing data are quite contradictory. This work is devoted to the study of the role of helicase activity of MLE in the regulation of gene expression in D. melanogaster. A site of intense MLE binding was found on chromosome 4 of D. melanogaster, in locus 102F. It was shown that MLE is a co-activator of expression of the Dyrk3, Toy, Sox102F, Shaven, and Fuss genes located in this locus. For this, the helicase activity of MLE is required. Genes whose expression depends on MLE are expressed at a high level in the nervous system of D. melanogaster and are essential for its proper development. The obtained data contributes to the study of potentially evolutionarily conserved functions of MLE.

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MLE/DHX9解旋酶活性调节黑腹果蝇4号染色体上一些组织特异性基因的表达。

黑腹蝶DHX9解旋酶及其同源物MLE参与不同阶段的基因表达。这两种解旋酶分别对人类和黑腹龙神经系统的形成和功能起重要作用。然而，关于DHX9和MLE解旋酶活性在基因表达调控中的作用研究甚少，现有数据也相当矛盾。本文旨在研究MLE解旋酶活性在黑腹菌基因表达调控中的作用。在4号染色体102F位点发现了一个MLE结合强烈的位点。结果表明，MLE是位于该位点的Dyrk3、Toy、Sox102F、Shaven和Fuss基因表达的共激活因子。为此，MLE的解旋酶活性是必需的。与MLE相关的基因在黑腹龙脑神经系统中表达水平较高，对其正常发育至关重要。获得的数据有助于研究MLE的潜在进化保守功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Doklady Biochemistry and Biophysics 生物-生化与分子生物学

CiteScore

1.60

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.