Integrated transcriptomic and metabolomic analyses reveal the molecular mechanisms regulating oil accumulation in Oenothera biennis L.

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products Pub Date : 2025-10-14 DOI:10.1016/j.indcrop.2025.122111

Peng Gao , Pinghui Li , Qi Qin , Tao Yang , Xiaoying Zhang , Jie Dong , Jinzhu Zhang , Daidi Che , Wuhua Zhang

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引用次数: 0

Abstract

Oenothera biennis L. is a multipurpose plant that can be valued for its seed oil rich in unsaturated fatty acids such as gamma-linolenic acid (GLA), exhibiting notable medicinal and economic importance. To elucidate the unclear mechanisms of its seed oil synthesis, this study examined morphological, biochemical, and molecular aspects of seed development and oil biosynthesis across nine stages. Oil accumulation exhibited four distinct phases: formation (0–12 d post-anthesis (DPA)), rapid accumulation (12–36 DPA), steady accumulation (36–48 DPA), and consumption (48–54 DPA). Morphological analysis showed capsules reached physiological maturity at 36 d, with peak dry weight and dimensional parameters. Linoleic acid constituted over 75 % of the total fatty acids, whereas GLA peaked at 8.18 % at 54 d. The oil bodies were first observed at 18 d via transmission electron microscopy, with a marked increase in number by 42 d and widespread cellular distribution. A total of 880 differentially expressed genes were identified through transcriptome sequencing, enriched in fatty acid synthesis, glycolysis, and plant hormone signaling pathways. Gene co-expression network analysis revealed 12 key genes (including ObFATA (Fatty acyl-ACP thioesterase A), ObFATB (Fatty acyl-ACP thioesterase B), and ObFAD2 (Fatty acid desaturase 2)) strongly correlated with fatty acid content. qRT-PCR confirmed significant upregulation of these genes during the rapid accumulation phase. Additionally, the seed oil exhibited potent antioxidant activity (2,2-diphenyl-1-picrylhydrazyl (DPPH)/2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)) and selective antibacterial effects against Staphylococcus aureus. These findings define the critical stages and molecular regulatory networks of oil biosynthesis in evening primrose seeds, offering a theoretical basis and genetic resources for molecular breeding of high-oil evening primrose varieties.

查看原文本刊更多论文

综合转录组学和代谢组学分析揭示了油品积累的分子机制。

双叶啤酒花是一种多用途植物，其籽油富含γ -亚麻酸（GLA）等不饱和脂肪酸，具有重要的药用和经济价值。为了阐明其种子油合成的机制，本研究从形态学、生化和分子方面研究了种子发育和油合成的九个阶段。原油成藏表现为4个阶段：形成阶段（开花后0 ~ 12 d）、快速积累阶段（12 ~ 36 d）、稳定积累阶段（36 ~ 48 d）和消耗阶段（48 ~ 54 d）。形态分析表明，36 d时蒴果达到生理成熟，具有最大的干重和尺寸参数。亚油酸占总脂肪酸的75% %以上，而GLA在54 d时达到8.18 %的峰值。在18 d时通过透射电镜首次观察到油体，其数量明显增加了42 d，细胞分布广泛。通过转录组测序共鉴定出880个差异表达基因，这些基因在脂肪酸合成、糖酵解和植物激素信号通路中富集。基因共表达网络分析显示，与脂肪酸含量密切相关的关键基因有ObFATA （Fatty酰基- acp硫酯酶A）、ObFATB （Fatty酰基- acp硫酯酶B）和ObFAD2（脂肪酸去饱和酶2）等12个。qRT-PCR证实了这些基因在快速积累阶段的显著上调。此外，该种子油还具有较强的抗氧化活性(2,2-二苯基-1-吡啶肼（DPPH)/2,2 ' -氮基-双（3-乙基苯并噻唑-6-磺酸）（ABTS））和对金黄色葡萄球菌的选择性抗菌作用。这些发现明确了月见草种子油脂生物合成的关键阶段和分子调控网络，为高油月见草品种的分子育种提供了理论依据和遗传资源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Industrial Crops and Products 农林科学-农业工程

CiteScore

9.50

自引率

8.50%

发文量

1518

审稿时长

43 days

期刊介绍： Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.