Soil-dwelling Naegleria enhances plant performance by stimulating beneficial bacterial functions in the rhizosphere.

IF 15.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Nature Communications Pub Date : 2025-10-13 DOI:10.1038/s41467-025-64139-x

Yang Yue,Zhihui Xu,Yijin Wang,Chen Liu,Shuo Sun,Xiangyu Ren,Qihui Lv,Jingchi Liu,Francisco Dini-Andreote,Wu Xiong,Qirong Shen

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引用次数: 0

Abstract

Soil protists play vital roles in influencing plant performance, yet their interactions with plant-beneficial bacteria are still poorly understood. Here, we examine how two soil protists (Naegleria sp. and Cercomonas sp.) affect the pathogen Ralstonia solanacearum, both on individual beneficial bacteria and within a synthetic microbial community (SynCom). Combining in vitro and pot experiments, we find that the SynCom together with Naegleria provided significantly greater suppression of the pathogen (enhanced suppressiveness by 74.29% compared to SynCom alone). Additionally, Naegleria increases SynCom biofilm biomass by 2.44 times. Population dynamics tracking revealed that Naegleria enriched Bacillus populations, leading to a positive correlation between Bacillus and Pseudomonas. Metatranscriptomics analysis shows upregulation of genes related to biofilm formation (such as epsA-O and tapA-sipW-tasA operon) and secondary metabolite biosynthesis (e.g., macrolactin H, bacillaene, and difficidin) in the presence of Naegleria. Our study demonstrates that Naegleria enhances plant health by predating on pathogens, promoting beneficial bacteria, and stimulating protective microbial functions in the rhizosphere.

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生活在土壤中的Naegleria通过刺激根际有益细菌的功能来提高植物的性能。

土壤原生生物在影响植物生长中起着至关重要的作用，但它们与植物有益菌的相互作用仍然知之甚少。在这里，我们研究了两种土壤原生生物（Naegleria sp.和Cercomonas sp.）如何影响病原菌，无论是对个体有益细菌还是在合成微生物群落（SynCom）内。结合体外和盆栽试验，我们发现SynCom和Naegleria对病原菌的抑制作用显著增强（与单独使用SynCom相比，抑制作用增强了74.29%）。此外，Naegleria使SynCom生物膜生物量增加2.44倍。种群动态追踪显示，纳格里氏菌富集芽孢杆菌种群，导致芽孢杆菌与假单胞菌正相关。超转录组学分析显示，在存在奈格杆菌的情况下，与生物膜形成相关的基因（如epsA-O和tapA-sipW-tasA操纵子）和次级代谢物生物合成（如大泌乳素H、芽胞杆菌烯和艰难梭菌素）上调。我们的研究表明，Naegleria通过捕食病原体、促进有益菌和刺激根际保护性微生物功能来促进植物健康。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Communications Biological Science Disciplines-

CiteScore

24.90

自引率

2.40%

发文量

6928

审稿时长

3.7 months

期刊介绍： Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.