Multiomics analyses of Reynoutria japonica unveil genomic landscape and biosynthesis of emodin anthraquinone in Polygonaceae

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products Pub Date : 2025-10-13 DOI:10.1016/j.indcrop.2025.122097

Lina Chen , Lin Sen , Aiping Deng , Die Hu , Yunting Zhang , Lijuan Sun , Fei Zeng , Yimei Liu , Xiao Huang , Juan Deng , Yunzhong Chen , Ling Gong , Kun Yu

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引用次数: 0

Abstract

Reynoutria japonica Houtt. (Polygonaceae), a medicinal plant valued for its anthraquinones, stilbenes, and flavonoids, is mainly cultivated for the extraction of resveratrol and emodin. However, the genome evolution and molecular mechanisms involved in the biosynthesis of emodin anthraquinone backbone remain elusive. Here, we present a comprehensive diploid genome assembly of 2.23 Gb, mapping 67144 protein-coding genes across 22 chromosomes using PacBio sequencing and Hi-C technology. Comparative genome analysis revealed a close genetic relationship with Rheum palmatum and R. hastatulus, and a shared dicotyledon γ-WGD event and a specific WGD event occurred 19 million years ago. Integrated analysis of transcriptomics, metabolomics and mass spectrometry imaging revealed 49 unique compounds, mainly including stilbenes, flavonoids and anthraquinones, and their significant accumulation trend in the root cortex. Three polyketone synthases (CHS1, CHS2, and PKS1) genes were characterized for the production of phloretin, emodin, and polydatin by heteroexpression and transgenic hairy roots of R. japonica, indicating their multifunctional roles in the biosynthesis of anthraquinone, stilbene and flavonoid. This study not only affords new viewpoints into genome evolution of Polygonaceae and anthraquinone biosynthesis, but also pave the way for functional genomic studies and genetic breeding of high resveratrol producing lines in R. japonica.

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多组学分析揭示了蓼科植物大黄素、蒽醌的基因组格局和生物合成

日本雷公酒。（蓼科）是一种药用植物，因其蒽醌、二苯乙烯和类黄酮而有价值，主要用于提取白藜芦醇和大黄素。然而，大黄素蒽醌骨架生物合成的基因组进化和分子机制尚不清楚。在这里，我们展示了2.23 Gb的全面二倍体基因组组装，使用PacBio测序和Hi-C技术绘制了22条染色体上的67144个蛋白质编码基因。基因组比较分析表明，该植物与掌叶大黄和hastatulus具有密切的亲缘关系，并在1900万年前发生了共同的双子叶γ-WGD事件和特定的WGD事件。转录组学、代谢组学和质谱成像综合分析发现了49种独特的化合物，主要包括芪类、黄酮类和蒽醌类，它们在根皮质中有显著的积累趋势。通过杂交表达和转基因粳米毛状根，鉴定了3个聚酮合成酶基因（CHS1、CHS2和PKS1）可产生根皮素、大黄素和多黄素，表明它们在合成蒽醌、二苯乙烯和黄酮类化合物中具有多种功能。本研究不仅为蓼科植物基因组进化和蒽醌生物合成提供了新的视角，而且为白藜芦醇高产系的功能基因组研究和遗传育种奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Industrial Crops and Products 农林科学-农业工程

CiteScore

9.50

自引率

8.50%

发文量

1518

审稿时长

43 days

期刊介绍： Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.