Decoding phenotypic signatures of Cylas formicarius Fab. resistance in a global sweetpotato (Ipomoea batatas [L.] Lam.) germplasm collection.

IF 4.1 2区生物学 Q1 PLANT SCIENCES

Frontiers in Plant Science Pub Date : 2025-09-26 eCollection Date: 2025-01-01 DOI:10.3389/fpls.2025.1625810

Alfredo Morales, Peiyong Ma, ZhaoDong Jia, Dania Rodríguez, Iván Javier Pastrana Vargas, Rosa Elena González, Osmany Molina, Alay Jiménez, Yuniel Rodríguez, Lilian Morales, Yoel Beovides, Orelvis Portal, Xiaofeng Bian

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引用次数: 0

Abstract

Introduction: Sweetpotato (Ipomoea batatas [L.] Lam.) is a critical global food crop that suffers devastating yield losses from the sweetpotato weevil (Cylas formicarius), especially in tropical regions where chemical control is often impractical. Breeding for stable resistance has been hindered by an insufficient characterization of reliable phenotypic markers across diverse genetic backgrounds.

Methods: We evaluated 731 accessions from Cuba's national sweetpotato collection, enriched with global varieties, to identify morphological traits associated with natural resistance to C. formicarius. Resistance and susceptibility were assessed through combined field and laboratory bioassays.

Results: Only 6.5% of the accessions demonstrated resistance (<10% infestation), while 80% were highly susceptible. Weak to moderate correlations linked resistance to smoother root surfaces (r = 0.31) and lighter flesh pigmentation (r = -0.38). The strongest correlation was observed with deeper tuberization (r = -0.72). Six Cuban genotypes combined agronomic viability (yield >10 t ha⁻¹) with resistance. Five of these employed deep tuberization as a physical escape mechanism, while one genotype, INIVIT B-25, exhibited shallow tuberization (mean depth 4.53 cm) yet maintained resistance, suggesting a biochemical defense strategy. Under controlled infestation, INIVIT B-2022 demonstrated the strongest antibiosis effect, suppressing adult emergence to just two individuals.

Discussion: Our study decodes key phenotypic signatures of weevil resistance, providing immediately actionable morphological traits for use in Caribbean breeding programs. The discovery of a resistant genotype with shallow roots indicates the presence of a non-escape, potentially biochemical resistance mechanism. This highlights the critical need for subsequent molecular studies to uncover the complementary genetic and biochemical bases of these defenses.

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密缕草表型特征的解码。全球甘薯（Ipomoea batatas）抗性的研究[j]。[林].)种质资源收集。

甘薯（Ipomoea batatas） [L。[Lam.])是一种重要的全球粮食作物，因甘薯象鼻虫（Cylas formicarius）而遭受毁灭性的产量损失，特别是在化学控制通常不切实际的热带地区。由于对不同遗传背景的可靠表型标记的描述不足，稳定抗性的育种一直受到阻碍。方法：我们对731份来自古巴国家甘薯收集品进行了评估，其中富含全球品种，以确定与对虫蜱虫自然抗性相关的形态特征。通过田间和实验室联合生物测定法评估抗性和敏感性。结果：仅有6.5%的菌株表现出抗药性（10毒毒毒血症）。其中5种采用深结核作为物理逃逸机制，而1种基因型INIVIT B-25表现出浅结核（平均深度4.53 cm），但仍保持抗性，表明存在生化防御策略。在虫害控制下，INIVIT B-2022表现出最强的抗菌效果，仅抑制2个个体的成虫羽化。讨论：我们的研究解码了象鼻虫抗性的关键表型特征，为加勒比育种计划提供了立即可操作的形态特征。具有浅根的抗性基因型的发现表明存在一种非逃逸的、潜在的生化抗性机制。这突出了后续分子研究的迫切需要，以揭示这些防御的互补遗传和生化基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Plant Science PLANT SCIENCES-

CiteScore

7.30

自引率

14.30%

发文量

4844

审稿时长

14 weeks

期刊介绍： In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches. Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.