{"title":"Lipidomic profiling reveals systemic serum lipid remodeling induced by dexamethasone in mice.","authors":"Aiping Zhang, Zhao Wang, Jianjian Ji","doi":"10.3389/fmolb.2025.1644637","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Dexamethasone is a commonly used glucocorticoid medication in pediatrics. However, dexamethasone is associated with some side effects, such as dyslipidemia. This study aimed to explore the effects of dexamethasone on the serum lipidome.</p><p><strong>Methods: </strong>We utilized Exactive Orbitrap-MS technology to assess the effects of dexamethasone intervention on serum lipids in mice.</p><p><strong>Results: </strong>Unbiased Principal Component Analysis (PCA) revealed that dexamethasone intervention significantly induced changes in serum lipids in mice, and after a 7-day washout period (equivalent to 28 drug half-lives), changes of lipids in the serum were still existed compared with those in the control groups. After 4 days of dexamethasone injection, significant changes were observed, including 16 increased lipids, and 1 decreased lipid in the serum compared with those in the control groups. After a 7-day washout period, some of lipids in the serum were still changed, including 5 increased lipids, such as Acylcarnitines (CAR), ceramides (Cer), diacylglycerophosphates (DG), lysophosphatidylglycerol (LPG) and phosphatidylglycerol (PG), 1 decreased lipid, hexosylceramides (HexCer), indicating dexamethasone may result in long-term changes of lipids in the serum.</p><p><strong>Discussion: </strong>In conclusion, utilizing a lipidomics method, we provide the first complete proof that dexamethasone intervention generates extensive modification of the serum lipidome.</p>","PeriodicalId":12465,"journal":{"name":"Frontiers in Molecular Biosciences","volume":"12 ","pages":"1644637"},"PeriodicalIF":3.9000,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12510821/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Molecular Biosciences","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3389/fmolb.2025.1644637","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Dexamethasone is a commonly used glucocorticoid medication in pediatrics. However, dexamethasone is associated with some side effects, such as dyslipidemia. This study aimed to explore the effects of dexamethasone on the serum lipidome.
Methods: We utilized Exactive Orbitrap-MS technology to assess the effects of dexamethasone intervention on serum lipids in mice.
Results: Unbiased Principal Component Analysis (PCA) revealed that dexamethasone intervention significantly induced changes in serum lipids in mice, and after a 7-day washout period (equivalent to 28 drug half-lives), changes of lipids in the serum were still existed compared with those in the control groups. After 4 days of dexamethasone injection, significant changes were observed, including 16 increased lipids, and 1 decreased lipid in the serum compared with those in the control groups. After a 7-day washout period, some of lipids in the serum were still changed, including 5 increased lipids, such as Acylcarnitines (CAR), ceramides (Cer), diacylglycerophosphates (DG), lysophosphatidylglycerol (LPG) and phosphatidylglycerol (PG), 1 decreased lipid, hexosylceramides (HexCer), indicating dexamethasone may result in long-term changes of lipids in the serum.
Discussion: In conclusion, utilizing a lipidomics method, we provide the first complete proof that dexamethasone intervention generates extensive modification of the serum lipidome.
期刊介绍：
Much of contemporary investigation in the life sciences is devoted to the molecular-scale understanding of the relationships between genes and the environment — in particular, dynamic alterations in the levels, modifications, and interactions of cellular effectors, including proteins. Frontiers in Molecular Biosciences offers an international publication platform for basic as well as applied research; we encourage contributions spanning both established and emerging areas of biology. To this end, the journal draws from empirical disciplines such as structural biology, enzymology, biochemistry, and biophysics, capitalizing as well on the technological advancements that have enabled metabolomics and proteomics measurements in massively parallel throughput, and the development of robust and innovative computational biology strategies. We also recognize influences from medicine and technology, welcoming studies in molecular genetics, molecular diagnostics and therapeutics, and nanotechnology.
Our ultimate objective is the comprehensive illustration of the molecular mechanisms regulating proteins, nucleic acids, carbohydrates, lipids, and small metabolites in organisms across all branches of life.
In addition to interesting new findings, techniques, and applications, Frontiers in Molecular Biosciences will consider new testable hypotheses to inspire different perspectives and stimulate scientific dialogue. The integration of in silico, in vitro, and in vivo approaches will benefit endeavors across all domains of the life sciences.
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