Intronic transposon insertion within the MYB transcription factor gene BjPur disturbs anthocyanin accumulation by inducing epigenetic modification in Brassica juncea.

IF 8.1 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2025-10-13 DOI:10.1111/nph.70637

Qiqi Cheng,Sihan Dong,Liping Guo,Shuhua Jiang,Na Yin,Donglan Sun,Fei Cheng,Changchun Yu,Zhengjie Wan

{"title":"Intronic transposon insertion within the MYB transcription factor gene BjPur disturbs anthocyanin accumulation by inducing epigenetic modification in Brassica juncea.","authors":"Qiqi Cheng,Sihan Dong,Liping Guo,Shuhua Jiang,Na Yin,Donglan Sun,Fei Cheng,Changchun Yu,Zhengjie Wan","doi":"10.1111/nph.70637","DOIUrl":null,"url":null,"abstract":"Transposable elements (TEs) account for about 50.36% of the Brassica juncea (B. juncea) genome, playing an important role in gene expression and genome evolution. However, the mechanisms by which host genes are influenced by TEs remain poorly understood. Our previous study revealed that the insertion of a 1268-bp DNA sequence into the intron of a MYB (BjPur) gene converts leaf color from purple to green in B. juncea. Here, we demonstrated that this insertion corresponds to a short interspersed nuclear element (SINE) retrotransposon, designated as Bjpur-SINE, which suppresses the expression of the Bjpur gene in green-leaved plants. Further analysis revealed that the insertion of Bjpur-SINE leads to increased DNA methylation at the BjPur locus, and the application of a DNA methylation inhibitor attenuated this transcriptional repression. Moreover, the knockout of two pivotal DNA methylation factors, BjDRM2 and BjAGO4, abolished Bjpur-SINE-mediated suppression and resulted in enhanced anthocyanin accumulation. Additionally, we observed alterations in repressive histone modifications at the BjPur locus in green-leaved B. juncea. Overall, our results confirmed that DNA and histone methylations synergistically participated in retrotransposon-mediated epigenetic silencing and provided insight into the mechanism by which intronic TEs regulated the expression of host genes.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"72 1","pages":""},"PeriodicalIF":8.1000,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"New Phytologist","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/nph.70637","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Transposable elements (TEs) account for about 50.36% of the Brassica juncea (B. juncea) genome, playing an important role in gene expression and genome evolution. However, the mechanisms by which host genes are influenced by TEs remain poorly understood. Our previous study revealed that the insertion of a 1268-bp DNA sequence into the intron of a MYB (BjPur) gene converts leaf color from purple to green in B. juncea. Here, we demonstrated that this insertion corresponds to a short interspersed nuclear element (SINE) retrotransposon, designated as Bjpur-SINE, which suppresses the expression of the Bjpur gene in green-leaved plants. Further analysis revealed that the insertion of Bjpur-SINE leads to increased DNA methylation at the BjPur locus, and the application of a DNA methylation inhibitor attenuated this transcriptional repression. Moreover, the knockout of two pivotal DNA methylation factors, BjDRM2 and BjAGO4, abolished Bjpur-SINE-mediated suppression and resulted in enhanced anthocyanin accumulation. Additionally, we observed alterations in repressive histone modifications at the BjPur locus in green-leaved B. juncea. Overall, our results confirmed that DNA and histone methylations synergistically participated in retrotransposon-mediated epigenetic silencing and provided insight into the mechanism by which intronic TEs regulated the expression of host genes.

查看原文本刊更多论文

MYB转录因子基因BjPur内含子转座子插入通过诱导表观遗传修饰干扰芥菜花青素积累。

转座因子（te）在芥菜（Brassica juncea， B. juncea）基因组中约占50.36%，在基因表达和基因组进化中发挥重要作用。然而，宿主基因受TEs影响的机制仍然知之甚少。我们之前的研究表明，将一个1268 bp的DNA序列插入到一个MYB （BjPur）基因的内含子中，可以使芥菜的叶子颜色从紫色变为绿色。在这里，我们证明了这个插入对应于一个短穿插核元件（sin）反转录转座子，被命名为Bjpur-SINE，它抑制绿叶植物中Bjpur基因的表达。进一步的分析表明，插入BjPur - sine导致BjPur位点的DNA甲基化增加，而DNA甲基化抑制剂的应用减弱了这种转录抑制。此外，敲除两个关键的DNA甲基化因子BjDRM2和BjAGO4，消除了bjpur - sin介导的抑制，导致花青素积累增强。此外，我们还观察到了绿叶芥菜BjPur位点抑制性组蛋白修饰的改变。总的来说，我们的研究结果证实了DNA和组蛋白甲基化协同参与了逆转录转座子介导的表观遗传沉默，并为内含子TEs调节宿主基因表达的机制提供了见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.