Reversible S-acylation of BONZAI1 orchestrates the internalization of immune receptors to balance plant development and immunity.

IF 24.1 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Plant Pub Date : 2025-10-10 DOI:10.1016/j.molp.2025.10.006

Xiaoshi Liu, Zhiying Wang, Shihui Li, Panpan Li, Meiqi Yuan, Xiaolin Lu, Chi Li, Yuewen Zheng, Zhendan Cao, Chuanliang Liu, Hongbo Li, Chao Wang, Caiji Gao, Chengwei Yang, Jianbin Lai

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引用次数: 0

Abstract

Plants have developed a precise immunity system for disease resistance and the receptors on the plasma membrane is controlled by endocytosis to modulate immune signaling, but endocytosis regulation in this process is unclear. Here we uncover that reversible S-acylation of BONZAI1 (BON1), a conserved copine family protein for development-immunity balance in Arabidopsis, contributes to accurate control of endocytosis. BON1 is targeted by S-acylation, a type of protein lipidation, for its localization on the plasma membrane and its function in development and immunity. Furthermore, the S-acylation status of BON1 affects its association with a light-chain clathrin subunit CLC3 and regulates endocytosis. Specifically, PAT14 facilitates the S-acylation of BON1, while ABAPT11 mediates its de-S-acylation. A physiological level of this reversible S-acylation of BON1 is essential for endocytosis and the internalization of immune receptors. Interestingly, salicylic acid enhances the ABAPT11-dependent de-S-acylation of BON1 for amplifying immunity signaling. This work on endocytosis regulation in plant development-immunity balance would assist with the improvement of crop yields and disease resistance.

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BONZAI1可逆s酰化调控免疫受体内化，平衡植物发育和免疫。

植物已经形成了精确的抗病免疫系统，质膜上的受体受胞吞作用控制，调节免疫信号，但胞吞作用在这一过程中的调控尚不清楚。本研究揭示了BONZAI1 （BON1）的可逆s酰化，BON1是拟南芥中一个保守的copine家族蛋白，用于发育-免疫平衡，有助于准确控制内吞噬。由于BON1在质膜上的定位及其在发育和免疫中的作用，BON1被s -酰化（一种蛋白质脂化）靶向。此外，BON1的s酰化状态影响其与轻链网格蛋白亚基CLC3的结合并调节内吞作用。具体来说，PAT14促进BON1的s酰化，而ABAPT11介导BON1的去s酰化。BON1可逆s酰化的生理水平对内吞作用和免疫受体的内化至关重要。有趣的是，水杨酸可以增强abapt11依赖性BON1的去s酰化，从而放大免疫信号。研究内吞作用在植物发育和免疫平衡中的调节作用，有助于提高作物产量和抗病性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Plant 植物科学-生化与分子生物学

CiteScore

37.60

自引率

2.20%

发文量

1784

审稿时长

1 months

期刊介绍： Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.