ROLE OF ANTIOXIDANT FOLIUM EXPOSURE ON OXIDATIVE SRESS IN A VALPROIC ACID-INDUCED ANIMAL MODEL OF AUTISM.

Abstract

Introduction: Autism spectrum disorder (ASD) is associated with increased levels of oxidative stress and decreased antioxidant capacity. The present study aimed to investigate the effects of different types of herbal antioxidant supplement Folium (F) - F. Relax, F. P53, F. pX, and F. Immuno (BAO Health Resources Corporation, USA) on reducing the severity of oxidative stress in a rat model of ASD induced by prenatal administration of valproic acid (VPA).

Methods: The study was conducted on male outbred white rats. Rats in the VPA treated groups (group - VPA), at 2 months of age, received intraperitoneally (17 mg/kg, for 21 days) different types of Folium. At the end of treatment, measurements were taken in both the control and experimental behaviorally characterized groups, including systemic blood pressure, heart rate, as well as oxidative (d-ROM) and antioxidant (PAT) statuses. Statistical analysis was performed using SigmaStat statistical software. Data for all parameters were analyzed statistically by one- or two-way ANOVA followed by post hoc comparisons. Student's t-test was used to compare the mean values of two independent groups.

Results: The results of behavioral studies showed that prenatal VPA treatment reduced social exploration, impaired social novelty preference, decreased anxiety, and increased locomotor activity. High blood pressure [systolic (SBP) and diastolic (DBP)] was observed in rats of the VPA group, along with an increased heart rate. VPA rats also showed elevated levels of free radicals (d-ROMs) and a higher oxidative stress index (OSI), indicating the presence of oxidative stress. Notably, treatment with different types of Folium revealed that: (i) administration of all these supplements restored blood pressure (systolic SBP and diastolic DBP) to the normal range; regarding heart rate, only F. Immuno did not produce a decrease; (ii) d-ROMs levels and the OSI in the VPA group were not significantly different from those in the VP+F. pX group.

Conclusion: Taken together, we provide evidence that prenatal administration of VPA to rats can induce ASD-like behavioral patterns accompanied by increased oxidative stress, as reflected by elevated levels of free radicals (d-ROMs) and a higher oxidative stress index (OSI). Based on the data obtained, it can be assumed that three of the four antioxidant supplements tested - F. Relax, F. P53, and F. Immuno, which showed positive effects on oxidative stress markers, may be suitable for use in individuals with ASD to alleviate oxidative stress and regulate blood pressure. However, to draw definitive conclusions, further research in collaboration with scientific research institutions and medical schools is required.