Prevalence and Antimicrobial Resistance of Klebsiella pneumoniae Isolated from Subclinical Mastitis in Selected Pure Dairy Cattle Breeds in Pakistan.

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2025-10-12 DOI:10.1007/s00284-025-04550-1

Sanam, Ijaz Ul Haq, Mustafa Kamal, Shehryar Khan, Irfan Khattak, Naimat Ullah Khan, Tariq Ali, Sana Riaz, Salvatore Massa, Tahir Usman

{"title":"Prevalence and Antimicrobial Resistance of Klebsiella pneumoniae Isolated from Subclinical Mastitis in Selected Pure Dairy Cattle Breeds in Pakistan.","authors":"Sanam, Ijaz Ul Haq, Mustafa Kamal, Shehryar Khan, Irfan Khattak, Naimat Ullah Khan, Tariq Ali, Sana Riaz, Salvatore Massa, Tahir Usman","doi":"10.1007/s00284-025-04550-1","DOIUrl":null,"url":null,"abstract":"Bovine mastitis caused by Klebsiella pneumoniae poses significant economic and health challenges in dairy production, particularly in its subclinical form. In this study, milk samples (n = 305) from different cattle breeds of Red Sindhi, Sahiwal, Cholistani, and Holstein Friesian were collected from six dairy farms in Pakistan. The prevalence of subclinical mastitis ranged from 42.3% in Red Sindhi to 77.0% in Sahiwal breeds, with an overall prevalence of 56.39%. The prevalence of K. pneumoniae was 11.8% (36/305), varying significantly among breeds: Cholistani (26.2%), Red Sindhi (17.3%), Holstein Friesian (5.0%), and Sahiwal (0%). Farm-specific prevalence ranged from 0% to 26.2%, underscoring the influence of local management practices. All tested microorganisms remained sensitive to cefotaxime, meropenem, and ciprofloxacin but revealed 100% resistance to ampicillin and 80.5% and 69.4% resistance to amoxicillin and streptomycin, respectively. Molecular analysis detected the blaTEM in 91.6% of isolates. These findings highlight significant breed and farm-level differences in subclinical mastitis and resistance patterns. The predominance of blaTEM and full susceptibility to third-generation cephalosporins suggest that local K. pneumoniae isolates may differ from global trends. Strengthened farm management and prudent antimicrobial use are essential to limit infections and safeguard both animal and public health.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 12","pages":"548"},"PeriodicalIF":2.6000,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00284-025-04550-1","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Bovine mastitis caused by Klebsiella pneumoniae poses significant economic and health challenges in dairy production, particularly in its subclinical form. In this study, milk samples (n = 305) from different cattle breeds of Red Sindhi, Sahiwal, Cholistani, and Holstein Friesian were collected from six dairy farms in Pakistan. The prevalence of subclinical mastitis ranged from 42.3% in Red Sindhi to 77.0% in Sahiwal breeds, with an overall prevalence of 56.39%. The prevalence of K. pneumoniae was 11.8% (36/305), varying significantly among breeds: Cholistani (26.2%), Red Sindhi (17.3%), Holstein Friesian (5.0%), and Sahiwal (0%). Farm-specific prevalence ranged from 0% to 26.2%, underscoring the influence of local management practices. All tested microorganisms remained sensitive to cefotaxime, meropenem, and ciprofloxacin but revealed 100% resistance to ampicillin and 80.5% and 69.4% resistance to amoxicillin and streptomycin, respectively. Molecular analysis detected the bla_TEM in 91.6% of isolates. These findings highlight significant breed and farm-level differences in subclinical mastitis and resistance patterns. The predominance of bla_TEM and full susceptibility to third-generation cephalosporins suggest that local K. pneumoniae isolates may differ from global trends. Strengthened farm management and prudent antimicrobial use are essential to limit infections and safeguard both animal and public health.

查看原文本刊更多论文

巴基斯坦部分纯奶牛亚临床乳腺炎分离肺炎克雷伯菌的流行及耐药性研究

由肺炎克雷伯菌引起的牛乳腺炎对乳制品生产构成了重大的经济和健康挑战，特别是在其亚临床形式下。在这项研究中，从巴基斯坦的六个奶牛场收集了红信德、萨希瓦尔、Cholistani和荷斯坦弗里西亚等不同牛品种的牛奶样本（n = 305）。亚临床乳腺炎的患病率从红信德犬的42.3%到萨希瓦尔犬的77.0%不等，总体患病率为56.39%。肺炎克雷伯菌患病率为11.8%(36/305)，各犬种间差异显著：Cholistani（26.2%）、Red Sindhi（17.3%）、Holstein Friesian（5.0%）和Sahiwal（0%）。具体农场的患病率从0%到26.2%不等，突出了当地管理做法的影响。所有检测的微生物对头孢噻肟、美罗培南和环丙沙星均保持敏感，但对氨苄西林的耐药性为100%，对阿莫西林和链霉素的耐药性分别为80.5%和69.4%。分子分析检测出91.6%的分离株。这些发现突出了亚临床乳腺炎和耐药模式在品种和农场水平上的显著差异。blaTEM的优势和对第三代头孢菌素的完全敏感性表明，当地肺炎克雷伯菌分离株可能与全球趋势不同。加强农场管理和谨慎使用抗微生物药物对于限制感染和保障动物和公众健康至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.