Understanding the mode of action of arsenic(III) on the fungus Neofusicoccum parvum: Target protein identification

IF 3.1 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2026-01-01 Epub Date: 2025-10-08 DOI:10.1016/j.ab.2025.115988

Andréa Engel , Florence Ferrari , Maude Meyer , Jean-Marc Strub , Martin Spichty , Christophe Bertsch , Christine Schaeffer-Reiss , Céline Tarnus , Sébastien Albrecht , Mary-Lorène Goddard

引用次数: 0

Abstract

Before it was banned, sodium arsenite was the unique fungicide able to control fungi associated with grapevine trunk diseases. However, its mode of action has not been fully elucidated yet. This study focuses on the identification of arsenic(III)-binding proteins using an arsenic-based fluorescent probe and an arsenic-based affinity chromatography. To the best of our knowledge, this is the first comparative study of these techniques to demonstrate their complementarity. Mainly cysteine-rich proteins were identified, the majority of which are involved in the infection process, in particular in plant cell wall degradation, host-pathogen interaction, adhesion and pathogenicity. These proteins could therefore be relevant targets for the development of new ways of grapevine trunk disease control.

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了解砷（III）对新褐菌的作用模式：靶蛋白鉴定。

在被禁止之前，亚砷酸钠是一种独特的杀菌剂，能够控制与葡萄树干疾病相关的真菌。然而，其作用方式尚未完全阐明。本研究的重点是利用砷基荧光探针和砷基亲和色谱法鉴定砷（III）结合蛋白。据我们所知，这是第一次对这些技术进行比较研究，以证明它们的互补性。主要鉴定出富含半胱氨酸的蛋白质，其中大部分参与了感染过程，特别是在植物细胞壁降解、宿主-病原体相互作用、粘附和致病性方面。因此，这些蛋白质可能是开发葡萄树干疾病控制新方法的相关靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.