Whole genome sequencing and comparative analysis of exopolysaccharide bioactivity from Lacticaseibacillus rhamnosus YT isolated from the feces of long-lived elderly individuals in Bama.

IF 8.5 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2025-10-08 DOI:10.1016/j.ijbiomac.2025.148199

Peng Yu, Jianbo Su, Yiping Liu, Xin Wang, Chenchen Zhang, Dawei Chen, Yujun Huang, Ruixia Gu, Chengran Guan

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Abstract

In this study, comprehensive whole-genome sequencing and comparative analysis of the exopolysaccharide (EPS) bioactivity of L. rhamnosus YT, a strain isolated from the feces of long-lived elderly individuals in Bama, Guangxi, were conducted. The genome of L. rhamnosus YT was revealed to be a circular chromosomal DNA of 2,961,987 bp, exhibiting a GC content of 46.73 % and encoding 2992 genes. Comparative genomic analysis with five other L. rhamnosus strains revealed significant collinearity in gene structure and function, yet differences were observed in gene inversions and the number of unique genes. Functional annotation demonstrated that L. rhamnosus YT possessed significant advantages in carbohydrate transport and metabolism, particularly with respect to key enzymes involved in the EPS synthesis pathway and sugar transport systems. Furthermore, a comparative assessment of the antioxidant activity, cholesterol adsorption capacity, and α-amylase inhibition activity between EPS produced by L. rhamnosus YT (EPS-YT) and L. rhamnosus GG (EPS-GG) showed that EPS-YT exhibited superior activities, especially in DPPH radical scavenging activity and cholesterol adsorption capacity. These findings provided profound insights into the genetic characteristics and adaptability of the L. rhamnosus YT, establishing a scientific foundation for its potential applications in probiotics and functional foods.

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巴马长寿老人粪便鼠李糖乳杆菌YT全基因组测序及胞外多糖生物活性比较分析。

本研究对广西巴马长寿老人粪便中分离的L. rhamnosus YT菌进行了全基因组测序，并对其胞外多糖（EPS）生物活性进行了比较分析。L. rhamnosus YT基因组为2961,987 bp的环状染色体DNA， GC含量为46.73 %，编码2992个基因。与其他5株鼠李糖菌株的基因组比较分析显示，该菌株在基因结构和功能上存在显著的共线性，但在基因倒置和独特基因数量上存在差异。功能注释表明，L. rhamnosus YT在碳水化合物运输和代谢方面具有显著优势，特别是在EPS合成途径和糖运输系统中涉及的关键酶方面。此外，通过对李糖糖YT （EPS-YT）和李糖糖GG （EPS-GG）生产的多糖的抗氧化活性、胆固醇吸附能力和α-淀粉酶抑制能力的比较，发现EPS-YT具有更强的抗氧化活性，特别是在DPPH自由基清除能力和胆固醇吸附能力方面。这些发现为深入了解鼠李糖YT的遗传特性和适应性提供了依据，为其在益生菌和功能食品中的潜在应用奠定了科学基础。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.