Investigation of plasmonic enhanced fluorescence effect induced from chips possessing numerous hotspots prepared by a facile process: Applied for the recognition of antigen-antibody reaction

IF 4.7 3区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Photochemistry and Photobiology A-chemistry Pub Date : 2025-10-01 DOI:10.1016/j.jphotochem.2025.116813

Gude Zhang , Hongzhong Hu , Xianling Piao , Yuhong Sun , Xian Wu Cheng , Xun Lu , Chunzhi Cui

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Abstract

Development of highly sensitive chips through a straightforward preparation process is a goal pursued by researchers and engineers. A common strategy involves the efficient utilization of plasmonic fields generated by noble metal nanostructures. In this study, we present a simple preparation process that yields sensor chips with numerous hotspot structures, achieved solely through ozone plasma treatment and silver (Ag) physical vapor deposition (PVD) on a photosensitive resin. Using these plasmonic-enhanced fluorescence (PEF) chips by attaching a polydiacetylene (PDA) probe material, an antigen-antibody interaction is successfully detected. Optimized Ag nanocluster arrays are demonstrated a 34-fold fluorescent enhancement comparing to the planar Ag nanostructure upon the concentration of the antigen myeloid progenitor cell inhibitory factor 1 (MPIF-1) is 1.25 ng/mL. According to analyses, it is found that the 34-fold fluorescent enhancement is attributed to the periodic structure and the hotspot effect. Based on this, the limit of detection (LOD) for the MPIF-1 is achieved the concentration of approximately 30 pg/mL.

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简易工艺制备多热点芯片诱导等离子体增强荧光效应的研究：用于抗原-抗体反应的识别

通过简单的制备过程开发高灵敏度芯片是研究人员和工程师追求的目标。一种常见的策略是有效利用贵金属纳米结构产生的等离子体场。在这项研究中，我们提出了一种简单的制备工艺，可以产生具有许多热点结构的传感器芯片，仅通过臭氧等离子体处理和银（Ag）物理气相沉积（PVD）在光敏树脂上实现。利用这些等离子体增强荧光（PEF）芯片，通过连接聚二乙炔（PDA）探针材料，成功地检测了抗原-抗体相互作用。当抗原髓系祖细胞抑制因子1 （MPIF-1）浓度为1.25 ng/mL时，优化后的银纳米簇阵列的荧光强度比平面银纳米结构增强34倍。分析发现，34倍的荧光增强是由周期性结构和热点效应引起的。在此基础上，MPIF-1的检出限（LOD）达到约30 pg/mL的浓度。

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来源期刊

Journal of Photochemistry and Photobiology A-chemistry 化学-物理化学

CiteScore

7.90

自引率

7.00%

发文量

580

审稿时长

48 days

期刊介绍： JPPA publishes the results of fundamental studies on all aspects of chemical phenomena induced by interactions between light and molecules/matter of all kinds. All systems capable of being described at the molecular or integrated multimolecular level are appropriate for the journal. This includes all molecular chemical species as well as biomolecular, supramolecular, polymer and other macromolecular systems, as well as solid state photochemistry. In addition, the journal publishes studies of semiconductor and other photoactive organic and inorganic materials, photocatalysis (organic, inorganic, supramolecular and superconductor). The scope includes condensed and gas phase photochemistry, as well as synchrotron radiation chemistry. A broad range of processes and techniques in photochemistry are covered such as light induced energy, electron and proton transfer; nonlinear photochemical behavior; mechanistic investigation of photochemical reactions and identification of the products of photochemical reactions; quantum yield determinations and measurements of rate constants for primary and secondary photochemical processes; steady-state and time-resolved emission, ultrafast spectroscopic methods, single molecule spectroscopy, time resolved X-ray diffraction, luminescence microscopy, and scattering spectroscopy applied to photochemistry. Papers in emerging and applied areas such as luminescent sensors, electroluminescence, solar energy conversion, atmospheric photochemistry, environmental remediation, and related photocatalytic chemistry are also welcome.