Differential aluminium toxicity in rice (Oryza sativa L.) landraces from Sikkim Himalaya: cellular Al-management determines tolerance

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-09-30 DOI:10.1016/j.plaphy.2025.110576

Poonam Chetry , Vijay Kumar , Pragati Yadav , Shailendra Goel , Shanti S. Sharma

{"title":"Differential aluminium toxicity in rice (Oryza sativa L.) landraces from Sikkim Himalaya: cellular Al-management determines tolerance","authors":"Poonam Chetry , Vijay Kumar , Pragati Yadav , Shailendra Goel , Shanti S. Sharma","doi":"10.1016/j.plaphy.2025.110576","DOIUrl":null,"url":null,"abstract":"<div><div>Aluminium (Al) toxicity comprises a constraint on acidic soils, substantially restricting plant productivity. We characterized the Al tolerance of ten unique rice (Oryza sativa L.) landraces from Sikkim Himalaya, known for rich diversity and cultivation of rice. Determination of root growth response to Al (0–100 μM) using activated charcoal staining revealed strong landrace specific variations in Al tolerance. Among tested landraces, Kalo tukmar and Krishna bhog proved most Al tolerant and sensitive, respectively. The charcoal staining assay offers an apparent advantage of greater accuracy and convenience over the conventional one for analysis of Al tolerance. Histochemical Al localization showed the degree of Al tolerance to be inversely related to that of root Al burden. The latter was associated with correspondingly increased loss of membrane integrity and level of lipid peroxidation. ROS (O2−<img> and H2O2) accumulation and transcript abundance of OsCAT and OsMSD1 are consistent with a link between redox metabolism and Al toxicity. A reduction in Al toxicity magnitude due to verapamil, a Ca2+ channel blocker, signifies the involvement of altered Ca2+ homeostasis in Al toxicity development. Differential OsNrat1 and OsALS1 transcript accumulation suggests a contrasting contribution of detoxification via vacuolar sequestration towards Al tolerance of two Al tolerant rice landraces namely, Kalo tukmar and Champasari. Thus, only the former seemed to rely on vacuolar sequestration. Upregulation of OsNrat1 with a concomitant downregulation of OsALS1 causing cytosolic Al retention likely contributed to the enhanced Al sensitivity of Krishna bhog. Findings indicate differences in the mechanism(s) of Al tolerance of tested rice landraces.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110576"},"PeriodicalIF":5.7000,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0981942825011040","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Aluminium (Al) toxicity comprises a constraint on acidic soils, substantially restricting plant productivity. We characterized the Al tolerance of ten unique rice (Oryza sativa L.) landraces from Sikkim Himalaya, known for rich diversity and cultivation of rice. Determination of root growth response to Al (0–100 μM) using activated charcoal staining revealed strong landrace specific variations in Al tolerance. Among tested landraces, Kalo tukmar and Krishna bhog proved most Al tolerant and sensitive, respectively. The charcoal staining assay offers an apparent advantage of greater accuracy and convenience over the conventional one for analysis of Al tolerance. Histochemical Al localization showed the degree of Al tolerance to be inversely related to that of root Al burden. The latter was associated with correspondingly increased loss of membrane integrity and level of lipid peroxidation. ROS (O₂⁻ and H₂O₂) accumulation and transcript abundance of OsCAT and OsMSD1 are consistent with a link between redox metabolism and Al toxicity. A reduction in Al toxicity magnitude due to verapamil, a Ca²⁺ channel blocker, signifies the involvement of altered Ca²⁺ homeostasis in Al toxicity development. Differential OsNrat1 and OsALS1 transcript accumulation suggests a contrasting contribution of detoxification via vacuolar sequestration towards Al tolerance of two Al tolerant rice landraces namely, Kalo tukmar and Champasari. Thus, only the former seemed to rely on vacuolar sequestration. Upregulation of OsNrat1 with a concomitant downregulation of OsALS1 causing cytosolic Al retention likely contributed to the enhanced Al sensitivity of Krishna bhog. Findings indicate differences in the mechanism(s) of Al tolerance of tested rice landraces.

查看原文本刊更多论文

锡金-喜马拉雅地区地方稻（Oryza sativa L.）的不同铝毒性：细胞铝管理决定耐受性

铝（Al）毒性包括对酸性土壤的约束，实质上限制了植物的生产力。我们对来自锡金-喜马拉雅地区的10个独特的地方品种（Oryza sativa L.）的铝耐受性进行了表征。用活性炭染色法测定根系生长对Al （0-100 μM）的响应，发现地方品种对Al的耐受性有很强的特异性差异。在测试的地方种族中，Kalo tukmar和Krishna bhog分别被证明是最耐受和敏感的。木炭染色法比传统的铝耐受性分析方法具有更高的准确性和便利性。组织化学铝定位表明，铝耐受性与根铝负荷量呈负相关。后者与相应增加的膜完整性损失和脂质过氧化水平有关。OsCAT和OsMSD1的ROS （O2−和H2O2）积累和转录丰度与氧化还原代谢与铝毒性之间的联系一致。维拉帕米（一种Ca2+通道阻滞剂）导致的铝毒性强度的降低，表明Ca2+稳态的改变参与了铝毒性的发展。不同的OsNrat1和OsALS1转录本积累表明，通过液泡固存解毒对Kalo tukmar和Champasari两种耐铝水稻的铝耐受性有不同的贡献。因此，似乎只有前者依赖于液泡封存。OsNrat1的上调伴随OsALS1的下调，导致细胞质Al滞留，可能是Krishna bhog对Al敏感性增强的原因。研究结果表明，不同地方水稻品种对铝的耐受性机制存在差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.