Biotransformation from rutin to quercetin in tomato by-products using almond β-glucosidase

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2026-02-01 Epub Date: 2025-10-06 DOI:10.1016/j.bej.2025.109955

Jared Mauricio López-Téllez, María del Pilar Cañizares-Macías

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Abstract

Within the circular economy, quercetin is a high-value polyphenol with significant food and pharmaceutical applications, which can be chemically or enzymatically produced from rutin. This study proposes an efficient enzymatic strategy using both free and immobilized β-glucosidase for rutin hydrolysis. When standard rutin was tested hydrolysis rates reached 80.69 ± 2.89 % (free enzyme) and 82.33 ± 3.45 % (immobilized enzyme), with conversion to quercetin at 49.14 ± 2.63 % and 55.22 ± 1.90 %, respectively. Application to tomato peels and seeds showed nonetheless very promising results with rutin hydrolysis rates between 29.14 ± 2.77 % (immobilized enzyme) and 35.86 ± 3.11 % (free enzyme) and quercetin conversion from 19.31 ± 2.16 % to 21.09 ± 3.63 %. Nonetheless, quercetin content and antioxidant capacity increased closely by 32.00 % and 18.00 %, respectively, after hydrolysis. These findings introduce a rapid, sustainable, and cost-effective enzymatic process for enhancing the bioactive potential of tomato by-products, providing a valuable tool for agro-industrial applications and contributing to the valorization of food waste.

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利用杏仁β-葡萄糖苷酶将番茄副产品中的芦丁转化为槲皮素

在循环经济中，槲皮素是一种高价值的多酚，具有重要的食品和制药应用，可以由芦丁化学或酶法生产。本研究提出了一种利用游离和固定化β-葡萄糖苷酶水解芦丁的有效方法。当标准测试芦丁水解率达到80.69 ±2.89  %(自由酶)和82.33 ±3.45  %(固定化酶),转换为槲皮素在49.14 ±2.63   %和55.22±1.90  %,分别。应用番茄皮和种子之间显示还是非常有前途的结果与芦丁水解率29.14 ±2.77   %(固定化酶)和35.86±3.11  %(自由酶)和槲皮素转换从19.31 ±2.16  % 21.09 ±3.63  %。水解后槲皮素含量和抗氧化能力分别提高了32.00 %和18.00 %。这些发现为提高番茄副产品的生物活性潜力提供了一种快速、可持续、经济的酶促过程，为农业工业应用提供了有价值的工具，并有助于食物垃圾的价值化。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.