Mirt2 alleviates LPS-induced inflammation of osteoblasts in alveolar bone destruction

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology Pub Date : 2025-10-09 DOI:10.1016/j.archoralbio.2025.106410

Yajie Wu , Zhifei Su , Bowen Zhang , Lei Cheng , Ziyou Wang , He Yuan , Jiyao Li

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引用次数: 0

Abstract

Objectives

The long noncoding RNA (lncRNA) myocardial infarction–associated transcript 2 (Mirt2) has been confirmed to affect several inflammatory diseases. This study was conducted to explore the functional mechanism of Mirt2 in inflammatory alveolar bone loss and its possibility of being a therapeutic target.

Design

The expression level and potential role of Mirt2 in chronic inflammatory alveolar bone loss in mouse models of periodontitis and periapical periodontitis were investigated using micro-CT and qPCR. The characteristics of Mirt2 were evaluated by FISH, qPCR, ELISA, and alkaline phosphatase staining to confirm its function and mechanism of action in inflammatory response.

Results

Mirt2 expression was significantly enriched in inflammatory alveolar bone diseases. Mirt2 expression increased upon LPS stimulation in MC3T3-E1 cells (P < 0.05), located at the cell cytoplasm. Mirt2 knockdown exacerbated the LPS-stimulated inflammatory response in MC3T3-E1 cells, whereas Mirt2 overexpression attenuated this effect and rescued LPS-impaired osteogenic differentiation.

Conclusions

lncRNA Mirt2 suggests a potential role in chronic inflammation–related bone loss, providing potential therapeutic target worthy of future investigation for inflammation-related bone loss.

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Mirt2可减轻lps诱导的成骨细胞在牙槽骨破坏中的炎症反应

目的长链非编码RNA （lncRNA）心肌梗死相关转录物2 （Mirt2）已被证实影响多种炎症性疾病。本研究旨在探讨Mirt2在炎症性牙槽骨丢失中的作用机制及其作为治疗靶点的可能性。设计采用micro-CT和qPCR方法研究Mirt2在牙周炎和根尖周炎小鼠慢性炎症性牙槽骨丢失中的表达水平及其潜在作用。通过FISH、qPCR、ELISA、碱性磷酸酶染色等方法评价Mirt2的特征，确认其在炎症反应中的功能和作用机制。结果smirt2在炎症性牙槽骨疾病中表达显著富集。MC3T3-E1细胞在LPS刺激下Mirt2表达增加（P <； 0.05），位于细胞质中。Mirt2敲低加重了lps刺激的MC3T3-E1细胞的炎症反应，而Mirt2过表达减弱了这种作用，并挽救了lps受损的成骨分化。结论slncrna Mirt2可能在慢性炎症相关性骨质流失中发挥潜在作用，为炎症相关性骨质流失提供了值得未来研究的潜在治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of oral biology 医学-牙科与口腔外科

CiteScore

5.10

自引率

3.30%

发文量

177

审稿时长

26 days

期刊介绍： Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including: Cell and molecular biology Molecular genetics Immunology Pathogenesis Cellular microbiology Embryology Syndromology Forensic dentistry