Fluorescence detection based on molecularly imprinted microspheres and graphene quantum dots/graphite carbon nitride for three tetracyclines determination in dairy products

IF 6.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

LWT - Food Science and Technology Pub Date : 2025-10-03 DOI:10.1016/j.lwt.2025.118585

Yinchuan Pan , Shudong Liu , Hao Zhang , Youming Shen , Jing Liu , Jianping Wang , Yanxia Gao , Ning Ma

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引用次数: 0

Abstract

Tetracyclines (TCs) residues in dairy products pose a potential threat to food safety and public health, necessitating sensitive and selective detection methods. In this study, a fluorescence-based sensing method was constructed by embedding nitrogen and phosphorus co-doped graphene quantum dots (NP-GQDs) into graphite carbon nitride (g-C₃N₄). The composite was subsequently combined with doxycycline-specific molecularly imprinted microspheres (MIM) to obtain MIM@NP-GQDs/g-C₃N₄. The composite-based assay exhibited a linear range of 2–50 ng/mL (R² > 0.99), with detection limits of 0.8–1.8 ng/mL for three TCs. The method showed excellent reproducibility, allowing up to six reuse cycles, and recovery rates in TC-free dairy samples ranged from 83.5 % to 96.7 % (RSD <5 %). This study demonstrated the application of MIM@NP-GQDs/g-C₃N₄ for TC detection in dairy products, offering a highly selective and reusable fluorescence-based detection strategy.

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基于分子印迹微球和石墨烯量子点/石墨氮化碳的荧光检测用于乳制品中三环类药物的测定

乳制品中四环素类残留对食品安全和公众健康构成潜在威胁，需要灵敏、选择性的检测方法。本研究将氮、磷共掺杂石墨烯量子点（NP-GQDs）嵌入到石墨碳氮（g-C3N4）中，构建了一种基于荧光的传感方法。该复合材料随后与强力霉素特异性分子印迹微球（MIM）结合得到MIM@NP-GQDs/g-C3N4。该方法线性范围为2 ~ 50 ng/mL (R2 > 0.99)，检出限为0.8 ~ 1.8 ng/mL。该方法重复性好，最多可重复使用6次，在不含tc的乳制品样品中回收率为83.5% ~ 96.7% （RSD < 5%）。本研究展示了MIM@NP-GQDs/g-C3N4在乳制品中TC检测中的应用，提供了一种高选择性和可重复使用的基于荧光的检测策略。

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来源期刊

LWT - Food Science and Technology 工程技术-食品科技

CiteScore

11.80

自引率

6.70%

发文量

1724

审稿时长

65 days

期刊介绍： LWT - Food Science and Technology is an international journal that publishes innovative papers in the fields of food chemistry, biochemistry, microbiology, technology and nutrition. The work described should be innovative either in the approach or in the methods used. The significance of the results either for the science community or for the food industry must also be specified. Contributions written in English are welcomed in the form of review articles, short reviews, research papers, and research notes. Papers featuring animal trials and cell cultures are outside the scope of the journal and will not be considered for publication.