RLCKs phosphorylate RopGEFs to control auxin-dependent Arabidopsis development.

IF 13.6 1区生物学 Q1 PLANT SCIENCES

Nature Plants Pub Date : 2025-10-10 DOI:10.1038/s41477-025-02111-9

Xiaojing Zhang,Hao Jiang,Guangqi Zhu,Fuhai Chen,Weichao Yuan,Yaqin Li,Wanqing Zhang,Dian Wu,Huili Liu,Hen-Ming Wu,Alice Y Cheung,Li-Zhen Tao

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引用次数: 0

Abstract

Auxin plays a critical role throughout plant development. We have established earlier that auxin activates the ROP (plant RHO GTPase) molecular switch, and that guanine nucleotide exchange factors ROPGEFs, which activate ROPs, are important regulators for myriad auxin-regulated processes. Here we show that auxin induces phosphorylation of RopGEF1 and that four receptor-like cytoplasmic kinases (RLCKs), named RopGEF1-activating kinases 1/2/3/4 (RAK1/2/3/4), mediate this process. We show that RAKs interact with RopGEFs and specifically phosphorylate S488 on RopGEF1, promoting its stability and recruitment to the cell membrane, and enhance ROP activation. Knockout of these RLCKs blocked auxin-stimulated RopGEF1 phosphorylation, reduced ROP activation and led to defects in PIN-FORMED (PIN)-mediated auxin distribution and multiple developmental processes. Phospho-mimic RopGEF1 (S488D) shows enhanced guanine nucleotide exchange activity in vitro, and its expression in rak quadruple mutants reverses their phenotypes. The RLCK-RopGEF linkage represents an important functional node and elucidates a critical missing link in ROP-meditated auxin signaling.

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RLCKs磷酸化ropgef以控制生长素依赖性拟南芥的发育。

生长素在植物发育过程中起着至关重要的作用。我们之前已经证实，生长素激活植物RHO GTPase分子开关，而激活ROP的鸟嘌呤核苷酸交换因子ROPGEFs是生长素调控过程的重要调节因子。本研究表明，生长素诱导RopGEF1磷酸化，四种被称为RopGEF1激活激酶1/2/3/4 （RAK1/2/3/4）的受体样细胞质激酶（RLCKs）介导了这一过程。我们发现RAKs与ropgef相互作用，特异性磷酸化RopGEF1上的S488，促进其稳定性和向细胞膜募集，并增强ROP的激活。敲除这些RLCKs阻断生长素刺激的RopGEF1磷酸化，降低ROP激活，导致PIN- formed （PIN - formed）介导的生长素分布和多种发育过程的缺陷。Phospho-mimic RopGEF1 （S488D）在体外显示出增强的鸟嘌呤核苷酸交换活性，其在rak四重突变体中的表达逆转了其表型。RLCK-RopGEF链接代表了一个重要的功能节点，并阐明了rop介导的生长素信号传导中一个关键的缺失环节。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Plants PLANT SCIENCES-

CiteScore

25.30

自引率

2.20%

发文量

196

期刊介绍： Nature Plants is an online-only, monthly journal publishing the best research on plants — from their evolution, development, metabolism and environmental interactions to their societal significance.