Two complementary NLRs from wild emmer wheat confer powdery mildew resistance.

IF 15.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Nature Communications Pub Date : 2025-10-10 DOI:10.1038/s41467-025-64052-3

Zuhuan Yang,Nannan Liu,Xiaoming Xie,Wenxin Wei,Yuhan Bai,Junna Sun,Wei Pan,Jiatian Yang,Weidong Wang,Xiaodong Xie,Muhammad Saqlain,Houyang Kang,Baoyun Li,Zhaorong Hu,Jinying Gou,Weilong Guo,Susheng Song,Jun Ma,Tzion Fahima,Qixin Sun,Lina Qiu,Yinghui Li,Chaojie Xie

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引用次数: 0

Abstract

Powdery mildew is a devastating disease that affects wheat yield and quality globally. Here, we identify a powdery mildew resistance locus MlIW39 from wild emmer wheat through map-based cloning, mutagenesis, and stable genetic transformation. Unlike many other cloned Pm genes, the MlIW39-mediated resistance is conferred by the combined effect of two complementary nucleotide-binding and leucine-rich repeat (NLR) genes, encoding a canonical coiled-coil (CC) type NLR protein (MlIW39-R1) and an atypical NLR protein (MlIW39-R2) with an unknown domain (CC-like), respectively. Overexpression of the NLR pair induces cell death in Nicotiana benthamiana, whereas MlIW39-R1 or MlIW39-R2 alone does not. The MlIW39-R1 and MlIW39-R2 proteins physically interact with each other. MlIW39-R1 and MlIW39-R2 likely originate independently and become neighborly located during evolution. Our findings shed light on the significance of NLR pairs in plant immunity and can facilitate wheat disease-resistance breeding using the developed MlIW39 introgression lines and functional marker.

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来自野生二粒小麦的两个互补nlr赋予白粉病抗性。

白粉病是一种影响全球小麦产量和品质的毁灭性疾病。本研究通过定位克隆、诱变和稳定的遗传转化，从野生二粒小麦中鉴定出一个抗白粉病基因座MlIW39。与许多其他克隆的Pm基因不同，mliw39介导的耐药是由两个互补的核苷酸结合和富含亮氨酸的重复序列（NLR）基因的联合作用赋予的，它们分别编码一个典型的卷曲卷曲（CC）型NLR蛋白（MlIW39-R1）和一个未知结构域（CC-样）的非典型NLR蛋白（MlIW39-R2）。NLR对的过表达诱导本烟细胞死亡，而MlIW39-R1或MlIW39-R2单独表达则不会。MlIW39-R1和MlIW39-R2蛋白之间存在物理相互作用。MlIW39-R1和MlIW39-R2可能是独立起源的，在进化过程中成为近邻。本研究结果揭示了NLR对在植物免疫中的重要意义，为利用已开发的MlIW39渗入系和功能标记进行小麦抗病育种提供了依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Communications Biological Science Disciplines-

CiteScore

24.90

自引率

2.40%

发文量

6928

审稿时长

3.7 months

期刊介绍： Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.