An NLR pair in the Pm68 locus confers powdery mildew resistance in durum and common wheat.

IF 15.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Nature Communications Pub Date : 2025-10-10 DOI:10.1038/s41467-025-64048-z

Huagang He,Qiulian Tang,Qianyuan Zhang,Shanying Zhu,Sijia Lv,Yinguang Bao,Jiabao Liang,Jiale Wang,Jin Wang,Hongxing Xu,Emile Cavalet-Giorsa,Simon G Krattinger,Hongjie Li,Chundu Wu,Anli Gao,Yajun Wang

{"title":"An NLR pair in the Pm68 locus confers powdery mildew resistance in durum and common wheat.","authors":"Huagang He,Qiulian Tang,Qianyuan Zhang,Shanying Zhu,Sijia Lv,Yinguang Bao,Jiabao Liang,Jiale Wang,Jin Wang,Hongxing Xu,Emile Cavalet-Giorsa,Simon G Krattinger,Hongjie Li,Chundu Wu,Anli Gao,Yajun Wang","doi":"10.1038/s41467-025-64048-z","DOIUrl":null,"url":null,"abstract":"Common wheat and durum wheat are widely cultivated cereal crops, both of which are infected by the obligate biotrophic pathogen Blumeria graminis f. sp. tritici (Bgt), causing powdery mildew. Recently, Pm68 gene was identified on the short arm of chromosome 2B in the Greek durum wheat line TRI 1796, conferring resistance to this disease. Here, we have cloned Pm68 from TRI 1796 using an integrated approach of genetic mapping, association analysis and PacBio sequencing. Transgenic assays demonstrate that Pm68 mediated resistance is controlled by a pair of genetically linked nucleotide-binding leucine-rich repeat (NLR)-encoding genes, Pm68-1 and Pm68-2. Transient expression assays in Nicotiana benthamiana leaves reveal that the activation of Pm68-1 is positively modulated by Pm68-2, likely through its N-terminal coiled-coil (CC)-like domain. Evolutionary analysis traces the origin of Pm68 to a specific wild emmer subpopulation. The introgression and transgenic wheat lines carrying this gene show no significant negative effects on major agronomic traits, highlighting the potential value of Pm68 for disease-resistant breeding programs of both durum wheat and common wheat.","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"2 1","pages":"9039"},"PeriodicalIF":15.7000,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature Communications","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.1038/s41467-025-64048-z","RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Common wheat and durum wheat are widely cultivated cereal crops, both of which are infected by the obligate biotrophic pathogen Blumeria graminis f. sp. tritici (Bgt), causing powdery mildew. Recently, Pm68 gene was identified on the short arm of chromosome 2B in the Greek durum wheat line TRI 1796, conferring resistance to this disease. Here, we have cloned Pm68 from TRI 1796 using an integrated approach of genetic mapping, association analysis and PacBio sequencing. Transgenic assays demonstrate that Pm68 mediated resistance is controlled by a pair of genetically linked nucleotide-binding leucine-rich repeat (NLR)-encoding genes, Pm68-1 and Pm68-2. Transient expression assays in Nicotiana benthamiana leaves reveal that the activation of Pm68-1 is positively modulated by Pm68-2, likely through its N-terminal coiled-coil (CC)-like domain. Evolutionary analysis traces the origin of Pm68 to a specific wild emmer subpopulation. The introgression and transgenic wheat lines carrying this gene show no significant negative effects on major agronomic traits, highlighting the potential value of Pm68 for disease-resistant breeding programs of both durum wheat and common wheat.

查看原文本刊更多论文

Pm68位点上的NLR对赋予硬粒小麦和普通小麦抗白粉病的能力。

普通小麦和硬粒小麦是广泛种植的谷类作物，它们都被专性生物营养病原体Blumeria graminis f. sp. tritici （Bgt）感染，引起白粉病。最近，在希腊硬粒小麦品系TRI 1796的2B染色体短臂上发现了Pm68基因，使其具有抗该病的能力。在这里，我们利用遗传作图、关联分析和PacBio测序的综合方法从TRI 1796中克隆出Pm68。转基因实验表明，Pm68介导的耐药是由一对基因连锁核苷酸结合富亮氨酸重复序列（NLR）编码基因Pm68-1和Pm68-2控制的。在烟叶中的瞬时表达分析表明，Pm68-1的激活可能通过其n端线圈（CC）样结构域被Pm68-2正向调节。进化分析将Pm68的起源追溯到一个特定的野生二聚体亚群。携带该基因的渐渗和转基因小麦品系对主要农艺性状没有显著的负面影响，这表明Pm68在硬粒小麦和普通小麦的抗病育种中具有潜在的价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Nature Communications Biological Science Disciplines-

CiteScore

24.90

自引率

2.40%

发文量

6928

审稿时长

3.7 months

期刊介绍： Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.